2oar

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[[Image:2oar.gif|left|200px]]
 
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{{Structure
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==Mechanosensitive Channel of Large Conductance (MscL)==
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|PDB= 2oar |SIZE=350|CAPTION= <scene name='initialview01'>2oar</scene>, resolution 3.50&Aring;
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<StructureSection load='2oar' size='340' side='right'caption='[[2oar]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AU:GOLD+ION'>AU</scene>
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<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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|GENE= mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [https://pdbe.org/2oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [https://www.ebi.ac.uk/pdbsum/2oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [http://www.ebi.ac.uk/pdbsum/2oar PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MSCL_MYCTA MSCL_MYCTA] Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell.[UniProtKB:P9WJN5]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oar_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].
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<div style="clear:both"></div>
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'''Mechanosensitive Channel of Large Conductance (MscL)'''
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha helices and a third cytoplasmic alpha helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.
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[[Category: Large Structures]]
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[[Category: Mechanosensitive Channels]]
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==About this Structure==
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[[Category: Mycobacterium tuberculosis H37Ra]]
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2OAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry 1MSL. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Chang G]]
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==Reference==
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[[Category: Lee AT]]
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Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]
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[[Category: Rees DC]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Spencer RH]]
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[[Category: Single protein]]
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[[Category: Steinbacher S]]
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[[Category: Chang, G.]]
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[[Category: Strop P]]
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[[Category: Lee, A T.]]
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[[Category: Rees, D C.]]
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[[Category: Spencer, R H.]]
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[[Category: Steinbacher, S.]]
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[[Category: Strop, P.]]
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[[Category: stretch activated ion channel mechanosensitive]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:05 2008''
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Current revision

Mechanosensitive Channel of Large Conductance (MscL)

PDB ID 2oar

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Proteopedia Page Contributors and Editors (what is this?)

OCA, Eric Martz, David S. Goodsell, Eran Hodis

Retrieved from "http://52.214.119.220/wiki/index.php/2oar"
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