2obr

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[[Image:2obr.gif|left|200px]]
 
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{{Structure
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==Crystal Structures of P Domain of Norovirus VA387==
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|PDB= 2obr |SIZE=350|CAPTION= <scene name='initialview01'>2obr</scene>, resolution 2.2&Aring;
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<StructureSection load='2obr' size='340' side='right'caption='[[2obr]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2obr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Norovirus Norovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OBR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2obr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obr OCA], [https://pdbe.org/2obr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2obr RCSB], [https://www.ebi.ac.uk/pdbsum/2obr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2obr ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2obr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obr OCA], [http://www.ebi.ac.uk/pdbsum/2obr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2obr RCSB]</span>
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[https://www.uniprot.org/uniprot/Q913Z3_9CALI Q913Z3_9CALI]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/2obr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2obr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Noroviruses are one of the major causes of nonbacterial gastroenteritis epidemics in humans. Recent studies on norovirus receptors show that different noroviruses recognize different human histo-blood group antigens (HBGAs), and eight receptor binding patterns of noroviruses have been identified. The P domain of the norovirus capsids is directly involved in this recognition. To determine the precise locations and receptor binding modes of HBGA carbohydrates on the viral capsids, a recombinant P protein of a GII-4 strain norovirus, VA387, was cocrystallized with synthetic type A or B trisaccharides. Based on complex crystal structures observed at a 2.0-A resolution, we demonstrated that the receptor binding site lies at the outermost end of the P domain and forms an extensive hydrogen-bonding network with the saccharide ligand. The A and B trisaccharides display similar binding modes, and the common fucose ring plays a key role in this interaction. The extensive interface between the two protomers in a P dimer also plays a crucial role in the formation of the receptor binding interface.
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'''Crystal Structures of P Domain of Norovirus VA387'''
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Structural basis for the recognition of blood group trisaccharides by norovirus.,Cao S, Lou Z, Tan M, Chen Y, Liu Y, Zhang Z, Zhang XC, Jiang X, Li X, Rao Z J Virol. 2007 Jun;81(11):5949-57. Epub 2007 Mar 28. PMID:17392366<ref>PMID:17392366</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2obr" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Noroviruses are one of the major causes of nonbacterial gastroenteritis epidemics in humans. Recent studies on norovirus receptors show that different noroviruses recognize different human histo-blood group antigens (HBGAs), and eight receptor binding patterns of noroviruses have been identified. The P domain of the norovirus capsids is directly involved in this recognition. To determine the precise locations and receptor binding modes of HBGA carbohydrates on the viral capsids, a recombinant P protein of a GII-4 strain norovirus, VA387, was cocrystallized with synthetic type A or B trisaccharides. Based on complex crystal structures observed at a 2.0-A resolution, we demonstrated that the receptor binding site lies at the outermost end of the P domain and forms an extensive hydrogen-bonding network with the saccharide ligand. The A and B trisaccharides display similar binding modes, and the common fucose ring plays a key role in this interaction. The extensive interface between the two protomers in a P dimer also plays a crucial role in the formation of the receptor binding interface.
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2OBR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Norovirus Norovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBR OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Structural basis for the recognition of blood group trisaccharides by norovirus., Cao S, Lou Z, Tan M, Chen Y, Liu Y, Zhang Z, Zhang XC, Jiang X, Li X, Rao Z, J Virol. 2007 Jun;81(11):5949-57. Epub 2007 Mar 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17392366 17392366]
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[[Category: Norovirus]]
[[Category: Norovirus]]
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[[Category: Single protein]]
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[[Category: Cao S]]
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[[Category: Cao, S.]]
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[[Category: Jiang X]]
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[[Category: Jiang, X.]]
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[[Category: Li X]]
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[[Category: Li, X.]]
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[[Category: Lou Z]]
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[[Category: Lou, Z.]]
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[[Category: Rao Z]]
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[[Category: Rao, Z.]]
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[[Category: Zhang XC]]
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[[Category: Zhang, X C.]]
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[[Category: crystal structure]]
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[[Category: norovirus va387]]
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[[Category: p domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:35 2008''
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Current revision

Crystal Structures of P Domain of Norovirus VA387

PDB ID 2obr

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