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| | ==NMR structure of Myxococcus xanthus antirepressor CarS1== | | ==NMR structure of Myxococcus xanthus antirepressor CarS1== |
| - | <StructureSection load='2kss' size='340' side='right' caption='[[2kss]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kss' size='340' side='right'caption='[[2kss]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kss]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25232 Atcc 25232]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kss]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">carS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34 ATCC 25232])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kss OCA], [http://pdbe.org/2kss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kss RCSB], [http://www.ebi.ac.uk/pdbsum/2kss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kss ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kss OCA], [https://pdbe.org/2kss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kss RCSB], [https://www.ebi.ac.uk/pdbsum/2kss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kss ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CARS_MYXXA CARS_MYXXA]] Involved in carotenoid biosynthesis. Antagonizes the transcriptional repressor proteins CarA and CarH by preventing their binding to DNA. Can also dissociate preformed CarA-DNA complexes. Does not bind DNA.<ref>PMID:11748235</ref> <ref>PMID:18315685</ref> | + | [https://www.uniprot.org/uniprot/CARS_MYXXA CARS_MYXXA] Involved in carotenoid biosynthesis. Antagonizes the transcriptional repressor proteins CarA and CarH by preventing their binding to DNA. Can also dissociate preformed CarA-DNA complexes. Does not bind DNA.<ref>PMID:11748235</ref> <ref>PMID:18315685</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Direct targeting of critical DNA-binding elements of a repressor by its cognate antirepressor is an effective means to sequester the repressor and remove a transcription initiation block. Structural descriptions for this, though often proposed for bacterial and phage repressor-antirepressor systems, are unavailable. Here, we describe the structural and functional basis of how the Myxococcus xanthus CarS antirepressor recognizes and neutralizes its cognate repressors to turn on a photo-inducible promoter. CarA and CarH repress the carB operon in the dark. CarS, produced in the light, physically interacts with the MerR-type winged-helix DNA-binding domain of these repressors leading to activation of carB. The NMR structure of CarS1, a functional CarS variant, reveals a five-stranded, antiparallel beta-sheet fold resembling SH3 domains, protein-protein interaction modules prevalent in eukaryotes but rare in prokaryotes. NMR studies and analysis of site-directed mutants in vivo and in vitro unveil a solvent-exposed hydrophobic pocket lined by acidic residues in CarS, where the CarA DNA recognition helix docks with high affinity in an atypical ligand-recognition mode for SH3 domains. Our findings uncover an unprecedented use of the SH3 domain-like fold for protein-protein recognition whereby an antirepressor mimics operator DNA in sequestering the repressor DNA recognition helix to activate transcription.
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| - | A bacterial antirepressor with SH3 domain topology mimics operator DNA in sequestering the repressor DNA recognition helix.,Leon E, Navarro-Aviles G, Santiveri CM, Flores-Flores C, Rico M, Gonzalez C, Murillo FJ, Elias-Arnanz M, Jimenez MA, Padmanabhan S Nucleic Acids Res. 2010 Aug;38(15):5226-41. Epub 2010 Apr 21. PMID:20410074<ref>PMID:20410074</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 2kss" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 25232]] | + | [[Category: Large Structures]] |
| - | [[Category: Elias-Arnanz, M]] | + | [[Category: Myxococcus xanthus]] |
| - | [[Category: Gonzalez, C]] | + | [[Category: Elias-Arnanz M]] |
| - | [[Category: Jimenez, M]] | + | [[Category: Gonzalez C]] |
| - | [[Category: Leon, E]] | + | [[Category: Jimenez M]] |
| - | [[Category: Navarro-Aviles, G]] | + | [[Category: Leon E]] |
| - | [[Category: Padmanabhan, S]] | + | [[Category: Navarro-Aviles G]] |
| - | [[Category: Activator]]
| + | [[Category: Padmanabhan S]] |
| - | [[Category: Antirepressor]]
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| - | [[Category: Carotenoid biosynthesis]]
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| - | [[Category: Protein]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription regulation]]
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| - | [[Category: Transcription regulator]]
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