2l0j

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==Solid State NMR structure of the M2 proton channel from Influenza A Virus in hydrated lipid bilayer==
==Solid State NMR structure of the M2 proton channel from Influenza A Virus in hydrated lipid bilayer==
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<StructureSection load='2l0j' size='340' side='right' caption='[[2l0j]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''>
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<StructureSection load='2l0j' size='340' side='right'caption='[[2l0j]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2l0j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/I72a2 I72a2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2l0j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Udorn/307/1972(H3N2)) Influenza A virus (A/Udorn/307/1972(H3N2))]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0J FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381517 I72A2])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0j OCA], [http://pdbe.org/2l0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0j RCSB], [http://www.ebi.ac.uk/pdbsum/2l0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0j ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0j OCA], [https://pdbe.org/2l0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0j RCSB], [https://www.ebi.ac.uk/pdbsum/2l0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>
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[https://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The M2 protein from the influenza A virus, an acid-activated proton-selective channel, has been the subject of numerous conductance, structural, and computational studies. However, little is known at the atomic level about the heart of the functional mechanism for this tetrameric protein, a His(37)-Trp(41) cluster. We report the structure of the M2 conductance domain (residues 22 to 62) in a lipid bilayer, which displays the defining features of the native protein that have not been attainable from structures solubilized by detergents. We propose that the tetrameric His(37)-Trp(41) cluster guides protons through the channel by forming and breaking hydrogen bonds between adjacent pairs of histidines and through specific interactions of the histidines with the tryptophan gate. This mechanism explains the main observations on M2 proton conductance.
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Insight into the mechanism of the influenza A proton channel from a structure in a lipid bilayer.,Sharma M, Yi M, Dong H, Qin H, Peterson E, Busath DD, Zhou HX, Cross TA Science. 2010 Oct 22;330(6003):509-12. PMID:20966252<ref>PMID:20966252</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2l0j" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Ion channels|Ion channels]]
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*[[Ion channels 3D structures|Ion channels 3D structures]]
*[[M2 protein|M2 protein]]
*[[M2 protein|M2 protein]]
== References ==
== References ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: I72a2]]
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[[Category: Large Structures]]
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[[Category: Busath, D D]]
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[[Category: Busath DD]]
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[[Category: Cross, T A]]
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[[Category: Cross TA]]
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[[Category: Dong, H]]
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[[Category: Dong H]]
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[[Category: Peterson, E]]
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[[Category: Peterson E]]
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[[Category: Qin, H]]
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[[Category: Qin H]]
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[[Category: Sharma, M]]
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[[Category: Sharma M]]
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[[Category: Yi, M]]
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[[Category: Yi M]]
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[[Category: Zhou, H X]]
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[[Category: Zhou HX]]
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[[Category: Conductance domain]]
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[[Category: Influenza]]
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[[Category: Lipid bilayer]]
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[[Category: M2 proton channel]]
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[[Category: Membrane domain]]
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[[Category: Solid state nmr]]
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[[Category: Transport protein]]
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[[Category: Viral protein]]
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Current revision

Solid State NMR structure of the M2 proton channel from Influenza A Virus in hydrated lipid bilayer

PDB ID 2l0j

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