6d7w

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==Cryo-EM structure of the mitochondrial calcium uniporter from N. fischeri at 3.8 Angstrom resolution==
==Cryo-EM structure of the mitochondrial calcium uniporter from N. fischeri at 3.8 Angstrom resolution==
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<StructureSection load='6d7w' size='340' side='right' caption='[[6d7w]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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<SX load='6d7w' size='340' side='right' viewer='molstar' caption='[[6d7w]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6d7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fischeri Aspergillus fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D7W FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6d7w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fischeri Aspergillus fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D7W FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6d80|6d80]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NFIA_105760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36630 Aspergillus fischeri])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d7w OCA], [https://pdbe.org/6d7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d7w RCSB], [https://www.ebi.ac.uk/pdbsum/6d7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d7w ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d7w OCA], [http://pdbe.org/6d7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d7w RCSB], [http://www.ebi.ac.uk/pdbsum/6d7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d7w ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/MCU_NEOFI MCU_NEOFI] Highly selective calcium channel localized to the inner mitochondrial membrane, which mediates calcium uptake into the mitochondrial matrix (PubMed:29995855). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed:29995855). Sufficient to operate as a pore-forming channel without the need of calcium-sensor or auxiliary subunit (PubMed:29995855).<ref>PMID:29995855</ref>
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The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel localized to the inner mitochondrial membrane. Here, we describe the structure of an MCU orthologue from the fungus Neosartorya fischeri (NfMCU) determined to 3.8 A resolution by phase-plate cryo-electron microscopy. The channel is a homotetramer with two-fold symmetry in its amino-terminal domain (NTD) that adopts a similar structure to that of human MCU. The NTD assembles as a dimer of dimers to form a tetrameric ring that connects to the transmembrane domain through an elongated coiled-coil domain. The ion-conducting pore domain maintains four-fold symmetry, with the selectivity filter positioned at the start of the pore-forming TM2 helix. The aspartate and glutamate sidechains of the conserved DIME motif are oriented towards the central axis and separated by one helical turn. The structure of NfMCU offers insights into channel assembly, selective calcium permeation, and inhibitor binding.
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Cryo-EM structure of a fungal mitochondrial calcium uniporter.,Nguyen NX, Armache JP, Lee C, Yang Y, Zeng W, Mootha VK, Cheng Y, Bai XC, Jiang Y Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0333-6. doi:, 10.1038/s41586-018-0333-6. PMID:29995855<ref>PMID:29995855</ref>
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==See Also==
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*[[Mitochondrial calcium uniporter|Mitochondrial calcium uniporter]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6d7w" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Aspergillus fischeri]]
[[Category: Aspergillus fischeri]]
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[[Category: Armache, J P]]
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[[Category: Large Structures]]
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[[Category: Bai, X C]]
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[[Category: Armache J-P]]
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[[Category: Cheng, Y]]
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[[Category: Bai XC]]
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[[Category: Nguyen, N X]]
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[[Category: Cheng Y]]
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[[Category: Calcium channel]]
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[[Category: Nguyen NX]]
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[[Category: Mitochondria]]
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[[Category: Transport protein]]
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Current revision

Cryo-EM structure of the mitochondrial calcium uniporter from N. fischeri at 3.8 Angstrom resolution

6d7w, resolution 3.80Å

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