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| | ==Backbone structure of human membrane protein TMEM141== | | ==Backbone structure of human membrane protein TMEM141== |
| - | <StructureSection load='2lor' size='340' side='right' caption='[[2lor]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lor' size='340' side='right'caption='[[2lor]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lor]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LOR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lor]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lom|2lom]], [[2lon|2lon]], [[2loo|2loo]], [[2lop|2lop]], [[2loq|2loq]], [[2los|2los]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TMEM141 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lor OCA], [https://pdbe.org/2lor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lor RCSB], [https://www.ebi.ac.uk/pdbsum/2lor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lor ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lor OCA], [http://pdbe.org/2lor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lor RCSB], [http://www.ebi.ac.uk/pdbsum/2lor PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lor ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TM141_HUMAN TM141_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Bayrhuber, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Choe, S]] | + | [[Category: Bayrhuber M]] |
| - | [[Category: Klammt, C]] | + | [[Category: Choe S]] |
| - | [[Category: Maslennikov, I]] | + | [[Category: Klammt C]] |
| - | [[Category: Riek, R]] | + | [[Category: Maslennikov I]] |
| - | [[Category: Helical bundle]]
| + | [[Category: Riek R]] |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
TM141_HUMAN
Publication Abstract from PubMed
Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.
Facile backbone structure determination of human membrane proteins by NMR spectroscopy.,Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S. Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626 doi:10.1038/nmeth.2033
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