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| | ==NMR structure of the Xanthomonas VirB7== | | ==NMR structure of the Xanthomonas VirB7== |
| - | <StructureSection load='2l4w' size='340' side='right' caption='[[2l4w]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2l4w' size='340' side='right'caption='[[2l4w]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l4w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanac Xanac]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L4W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L4W FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ov5|3ov5]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XAC2622 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4w OCA], [https://pdbe.org/2l4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l4w RCSB], [https://www.ebi.ac.uk/pdbsum/2l4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4w OCA], [http://pdbe.org/2l4w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l4w RCSB], [http://www.ebi.ac.uk/pdbsum/2l4w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8PJB3_XANAC Q8PJB3_XANAC] |
| - | Type IV secretion systems (T4SS) are used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Translocation across the outer membrane is achieved via a ringed tetradecameric outer membrane complex made up of a small VirB7 lipoprotein (normally 30 to 45 residues in the mature form) and the C-terminal domains of the VirB9 and VirB10 subunits. Several species from the genera of Xanthomonas phytopathogens possess an uncharacterized type IV secretion system with some distinguishing features, one of which is an unusually large VirB7 subunit (118 residues in the mature form). Here, we report the NMR and 1.0 A X-ray structures of the VirB7 subunit from Xanthomonas citri subsp. citri (VirB7(XAC2622)) and its interaction with VirB9. NMR solution studies show that residues 27-41 of the disordered flexible N-terminal region of VirB7(XAC2622) interact specifically with the VirB9 C-terminal domain, resulting in a significant reduction in the conformational freedom of both regions. VirB7(XAC2622) has a unique C-terminal domain whose topology is strikingly similar to that of N0 domains found in proteins from different systems involved in transport across the bacterial outer membrane. We show that VirB7(XAC2622) oligomerizes through interactions involving conserved residues in the N0 domain and residues 42-49 within the flexible N-terminal region and that these homotropic interactions can persist in the presence of heterotropic interactions with VirB9. Finally, we propose that VirB7(XAC2622) oligomerization is compatible with the core complex structure in a manner such that the N0 domains form an extra layer on the perimeter of the tetradecameric ring.
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| - | A Component of the Xanthomonadaceae Type IV Secretion System Combines a VirB7 Motif with a N0 Domain Found in Outer Membrane Transport Proteins.,Souza DP, Andrade MO, Alvarez-Martinez CE, Arantes GM, Farah CS, Salinas RK PLoS Pathog. 2011 May;7(5):e1002031. Epub 2011 May 12. PMID:21589901<ref>PMID:21589901</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 2l4w" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Xanac]] | + | [[Category: Large Structures]] |
| - | [[Category: Farah, C S]] | + | [[Category: Xanthomonas citri pv. citri str. 306]] |
| - | [[Category: Salinas, R K]] | + | [[Category: Farah CS]] |
| - | [[Category: Souza, D P]] | + | [[Category: Salinas RK]] |
| - | [[Category: Bacterial outer membrane]] | + | [[Category: Souza DP]] |
| - | [[Category: Lipoprotein]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: N0 domain]]
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| - | [[Category: Protein transport]]
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| - | [[Category: Type iv secretion system]]
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| - | [[Category: Virb7]]
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| - | [[Category: Xanthomona]]
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