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| | ==Structure/function of the LBR Tudor domain== | | ==Structure/function of the LBR Tudor domain== |
| - | <StructureSection load='2l8d' size='340' side='right' caption='[[2l8d]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2l8d' size='340' side='right'caption='[[2l8d]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l8d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l8d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8D FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LBR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8d OCA], [http://pdbe.org/2l8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8d RCSB], [http://www.ebi.ac.uk/pdbsum/2l8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8d OCA], [https://pdbe.org/2l8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8d RCSB], [https://www.ebi.ac.uk/pdbsum/2l8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LBR_CHICK LBR_CHICK]] Anchors the lamina and the heterochromatin to the inner nuclear membrane. Can interact with chromodomain proteins. | + | [https://www.uniprot.org/uniprot/LBR_CHICK LBR_CHICK] Anchors the lamina and the heterochromatin to the inner nuclear membrane. Can interact with chromodomain proteins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chick]] | + | [[Category: Gallus gallus]] |
| - | [[Category: Edlich, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Georgatos, S D]] | + | [[Category: Edlich C]] |
| - | [[Category: Giannios, I]] | + | [[Category: Georgatos SD]] |
| - | [[Category: Liokatis, S]] | + | [[Category: Giannios I]] |
| - | [[Category: Politou, A S]] | + | [[Category: Liokatis S]] |
| - | [[Category: Sattler, M]] | + | [[Category: Politou AS]] |
| - | [[Category: Soupsana, K]] | + | [[Category: Sattler M]] |
| - | [[Category: Dna binding protein]]
| + | [[Category: Soupsana K]] |
| Structural highlights
Function
LBR_CHICK Anchors the lamina and the heterochromatin to the inner nuclear membrane. Can interact with chromodomain proteins.
Publication Abstract from PubMed
Lamin B receptor (LBR) is a polytopic protein of the nuclear envelope thought to connect the inner nuclear membrane with the underlying nuclear lamina and peripheral heterochromatin. To better understand the function of this protein, we have examined in detail its nucleoplasmic region, which is predicted to harbor a Tudor domain (LBR-TD). Structural analysis by multidimensional NMR spectroscopy establishes that LBR-TD indeed adopts a classical beta-barrel Tudor fold in solution, which, however, features an incomplete aromatic cage. Removal of LBR-TD renders LBR more mobile at the plane of the nuclear envelope, but the isolated module does not bind to nuclear lamins, heterochromatin proteins (MeCP2), and nucleosomes, nor does it associate with methylated Arg/Lys residues through its aromatic cage. Instead, LBR-TD exhibits tight and stoichiometric binding to the "histone-fold" region of unassembled, free histone H3, suggesting an interesting role in histone assembly. Consistent with such a role, robust binding to native nucleosomes is observed when LBR-TD is extended toward its carboxyl terminus, to include an area rich in Ser-Arg residues. The Ser-Arg region, alone or in combination with LBR-TD, binds both unassembled and assembled H3/H4 histones, suggesting that the TD/RS interface may operate as a "histone chaperone-like platform."
Solution structure and molecular interactions of lamin B receptor tudor domain.,Liokatis S, Edlich C, Soupsana K, Giannios I, Panagiotidou P, Tripsianes K, Sattler M, Georgatos SD, Politou AS J Biol Chem. 2012 Jan 6;287(2):1032-42. Epub 2011 Nov 3. PMID:22052904[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liokatis S, Edlich C, Soupsana K, Giannios I, Panagiotidou P, Tripsianes K, Sattler M, Georgatos SD, Politou AS. Solution structure and molecular interactions of lamin B receptor tudor domain. J Biol Chem. 2012 Jan 6;287(2):1032-42. Epub 2011 Nov 3. PMID:22052904 doi:10.1074/jbc.M111.281303
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