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2lkq

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NMR structure of the lambda 5 22-45 peptide

Structural highlights

2lkq is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

IGLL1_HUMAN Defects in IGLL1 are the cause of agammaglobulinemia type 2 (AGM2) [MIM:613500. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.

Function

IGLL1_HUMAN Critical for B-cell development.^[1]

Publication Abstract from PubMed

During B cell differentiation in the bone marrow, the expression and activation of the pre-B cell receptor (pre-BCR) constitutes a crucial checkpoint for B cell development. Both constitutive and ligand-dependent pre-BCR activation modes have been described. The pre-BCR is constituted of an immunoglobulin heavy chain (Igmu) and of a surrogate light chain (SLC) composed of the invariant lambda5 and VpreB proteins. We previously showed that Galectin-1 (GAL1), produced by bone marrow stromal cells, is a pre-BCR ligand that induces receptor clustering, leading to efficient pre-BII cell proliferation and differentiation. GAL1 interacts with the pre-BCR via the unique region of lambda5 (lambda5-UR). Here we investigated the solution structure of a minimal lambda5-UR motif that interacts with GAL1. This motif adopts a stable helical conformation that docks onto a GAL1 hydrophobic surface adjacent to its carbohydrate-binding site (CBS). We identified key hydrophobic residues from the lambda5-UR as crucial for the interaction with GAL1 and for pre-BCR clustering. These residues involved in GAL1-induced pre-BCR activation are different to those essential for autonomous receptor activation. Overall, our results indicate that constitutive and ligand-induced pre-BCR activation could occur in a complementary manner.

Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation.,Elantak L, Espeli M, Boned A, Bornet O, Bonzi J, Gauthier L, Feracci M, Roche P, Guerlesquin F, Schiff C J Biol Chem. 2012 Nov 2. PMID:23124203^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Minegishi Y, Coustan-Smith E, Wang YH, Cooper MD, Campana D, Conley ME. Mutations in the human lambda5/14.1 gene result in B cell deficiency and agammaglobulinemia. J Exp Med. 1998 Jan 5;187(1):71-7. PMID:9419212
↑ Elantak L, Espeli M, Boned A, Bornet O, Bonzi J, Gauthier L, Feracci M, Roche P, Guerlesquin F, Schiff C. Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation. J Biol Chem. 2012 Nov 2. PMID:23124203 doi:http://dx.doi.org/10.1074/jbc.M112.395152

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lkq"

@@ Line 1: / Line 1: @@
 ==NMR structure of the lambda 5 22-45 peptide==
-<StructureSection load='2lkq' size='340' side='right' caption='[[2lkq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2lkq' size='340' side='right'caption='[[2lkq]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lkq]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LKQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKQ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkq OCA], [http://pdbe.org/2lkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lkq RCSB], [http://www.ebi.ac.uk/pdbsum/2lkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkq ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkq OCA], [https://pdbe.org/2lkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkq RCSB], [https://www.ebi.ac.uk/pdbsum/2lkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkq ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/IGLL1_HUMAN IGLL1_HUMAN]] Defects in IGLL1 are the cause of agammaglobulinemia type 2 (AGM2) [MIM:[http://omim.org/entry/613500 613500]]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
+[https://www.uniprot.org/uniprot/IGLL1_HUMAN IGLL1_HUMAN] Defects in IGLL1 are the cause of agammaglobulinemia type 2 (AGM2) [MIM:[https://omim.org/entry/613500 613500]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
 == Function ==
-[[http://www.uniprot.org/uniprot/IGLL1_HUMAN IGLL1_HUMAN]] Critical for B-cell development.<ref>PMID:9419212</ref>
+[https://www.uniprot.org/uniprot/IGLL1_HUMAN IGLL1_HUMAN] Critical for B-cell development.<ref>PMID:9419212</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Boned, A]]
+[[Category: Homo sapiens]]
-[[Category: Bornet, O]]
+[[Category: Large Structures]]
-[[Category: Breton, C]]
+[[Category: Boned A]]
-[[Category: Elantak, L]]
+[[Category: Bornet O]]
-[[Category: Espeli, M]]
+[[Category: Breton C]]
-[[Category: Feracci, M]]
+[[Category: Elantak L]]
-[[Category: Guerlesquin, F]]
+[[Category: Espeli M]]
-[[Category: Roche, P]]
+[[Category: Feracci M]]
-[[Category: Schiff, C]]
+[[Category: Guerlesquin F]]
-[[Category: B cell development]]
+[[Category: Roche P]]
-[[Category: Immune system]]
+[[Category: Schiff C]]
-[[Category: Pre-bcr]]

2lkq

From Proteopedia

Current revision

NMR structure of the lambda 5 22-45 peptide

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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