6e3a
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==tRNA 2'-phosphotransferase== | |
| + | <StructureSection load='6e3a' size='340' side='right'caption='[[6e3a]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6e3a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus_ATCC_27405 Acetivibrio thermocellus ATCC 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E3A FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=HQG:[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]+[(2~{R},3~{S},4~{R},5~{R})-3,4-bis(oxidanyl)-5-phosphonooxy-oxolan-2-yl]methyl+hydrogen+phosphate'>HQG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e3a OCA], [https://pdbe.org/6e3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e3a RCSB], [https://www.ebi.ac.uk/pdbsum/6e3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e3a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/KPTA_ACET2 KPTA_ACET2] Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR>P). May function as an ADP-ribosylase.[HAMAP-Rule:MF_00299] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Tpt1 is an essential agent of fungal tRNA splicing that removes the 2'-PO4 at the splice junction generated by fungal tRNA ligase. Tpt1 catalyzes a unique two-step reaction whereby the 2'-PO4 attacks NAD(+) to form an RNA-2'-phospho-ADP-ribosyl intermediate that undergoes transesterification to yield 2'-OH RNA and ADP-ribose-1'',2''-cyclic phosphate products. Because Tpt1 is inessential in exemplary bacterial and mammalian taxa, Tpt1 is seen as an attractive antifungal target. Here we report a 1.4 A crystal structure of Tpt1 in a product-mimetic complex with ADP-ribose-1''-phosphate in the NAD(+) site and pAp in the RNA site. The structure reveals how Tpt1 recognizes a 2'-PO4 RNA splice junction and the mechanism of RNA phospho-ADP-ribosylation. This study also provides evidence that a bacterium has an endogenous phosphorylated substrate with which Tpt1 reacts. | ||
| - | + | Structure of tRNA splicing enzyme Tpt1 illuminates the mechanism of RNA 2'-PO4 recognition and ADP-ribosylation.,Banerjee A, Munir A, Abdullahu L, Damha MJ, Goldgur Y, Shuman S Nat Commun. 2019 Jan 15;10(1):218. doi: 10.1038/s41467-018-08211-9. PMID:30644400<ref>PMID:30644400</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6e3a" style="background-color:#fffaf0;"></div> |
| - | [[Category: Banerjee | + | == References == |
| - | [[Category: Shuman | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Acetivibrio thermocellus ATCC 27405]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Banerjee A]] | ||
| + | [[Category: Goldgur Y]] | ||
| + | [[Category: Shuman S]] | ||
Current revision
tRNA 2'-phosphotransferase
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