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2opc

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[[Image:2opc.gif|left|200px]]
 
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{{Structure
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==Structure of Melampsora lini avirulence protein, AvrL567-A==
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|PDB= 2opc |SIZE=350|CAPTION= <scene name='initialview01'>2opc</scene>, resolution 1.43&Aring;
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<StructureSection load='2opc' size='340' side='right'caption='[[2opc]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>
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<table><tr><td colspan='2'>[[2opc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OPC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43&#8491;</td></tr>
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|GENE= AvrL567-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5261 Melampsora lini])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2opc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opc OCA], [https://pdbe.org/2opc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2opc RCSB], [https://www.ebi.ac.uk/pdbsum/2opc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2opc ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2opc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opc OCA], [http://www.ebi.ac.uk/pdbsum/2opc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2opc RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q6R661_MELLI Q6R661_MELLI]
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== Evolutionary Conservation ==
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'''Structure of Melampsora lini avirulence protein, AvrL567-A'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/2opc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2opc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
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==About this Structure==
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The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein.,Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816<ref>PMID:17329816</ref>
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2OPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17329816 17329816]
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</div>
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<div class="pdbe-citations 2opc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Melampsora lini]]
[[Category: Melampsora lini]]
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[[Category: Single protein]]
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[[Category: Catanzariti AM]]
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[[Category: Catanzariti, A M.]]
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[[Category: Dodds PN]]
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[[Category: Dodds, P N.]]
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[[Category: Ellis JG]]
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[[Category: Ellis, J G.]]
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[[Category: Forwood JK]]
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[[Category: Forwood, J K.]]
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[[Category: Guncar G]]
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[[Category: Guncar, G.]]
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[[Category: Kobe B]]
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[[Category: Kobe, B.]]
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[[Category: Teh T]]
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[[Category: Teh, T.]]
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[[Category: Wang CI]]
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[[Category: Wang, C I.]]
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[[Category: avrl567-a]]
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[[Category: cobalt]]
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[[Category: crystallization]]
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[[Category: plant disease resistance]]
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[[Category: single-wavelength anomalous dispersion (sad)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:08 2008''
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Current revision

Structure of Melampsora lini avirulence protein, AvrL567-A

PDB ID 2opc

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