6h6k
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6h6k is ON HOLD Authors: Nikonov, O., Kravchenko, O., Nevskaya, N., Stolboushkina, E., Gabdulkhakov, A., Garber, M., Nikonov, S. Description: The s...) |
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of the FKR mutant of the archaeal translation initiation factor 2 gamma subunit in complex with GDPCP, obtained in the absence of magnesium salts in the crystallization solution.== |
| + | <StructureSection load='6h6k' size='340' side='right'caption='[[6h6k]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6h6k]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H6K FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eif2g, SSO0412 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h6k OCA], [http://pdbe.org/6h6k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h6k RCSB], [http://www.ebi.ac.uk/pdbsum/6h6k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h6k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/IF2G_SULSO IF2G_SULSO]] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the gamma subunit of archaeal translation initiation factor 2 (aIF2) from Sulfolobus solfataricus (SsoIF2gamma) was determined in complex with GDPCP (a GTP analog). Crystals were obtained in the absence of magnesium ions in the crystallization solution. They belonged to space group P1, with five molecules in the unit cell. Four of these molecules are related in pairs by a common noncrystallographic twofold symmetry axis, while the fifth has no symmetry equivalent. Analysis of the structure and its comparison with other known aIF2 gamma-subunit structures in the GTP-bound state show that (i) the magnesium ion is necessary for the formation and the maintenance of the active form of SsoIF2gamma and (ii) in addition to the two previously known structural switches 1 and 2, eukaryotic translation initiation factor 2 (eIF2) and aIF2 molecules have another flexible region (switch 3), the function of which may consist of initiation of the hydrolysis of GTP and the removal of e/aIF2 from the ribosome after codon-anticodon recognition. | ||
| - | + | The third structural switch in the archaeal translation initiation factor 2 (aIF2) molecule and its possible role in the initiation of GTP hydrolysis and the removal of aIF2 from the ribosome.,Nikonov O, Kravchenko O, Nevskaya N, Stolboushkina E, Garber M, Nikonov S Acta Crystallogr D Struct Biol. 2019 Apr 1;75(Pt 4):392-399. doi:, 10.1107/S2059798319002304. Epub 2019 Mar 28. PMID:30988256<ref>PMID:30988256</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6h6k" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sacs2]] | ||
| + | [[Category: Gabdulkhakov, A]] | ||
[[Category: Garber, M]] | [[Category: Garber, M]] | ||
[[Category: Kravchenko, O]] | [[Category: Kravchenko, O]] | ||
| - | [[Category: Stolboushkina, E]] | ||
| - | [[Category: Nikonov, O]] | ||
| - | [[Category: Gabdulkhakov, A]] | ||
[[Category: Nevskaya, N]] | [[Category: Nevskaya, N]] | ||
| + | [[Category: Nikonov, O]] | ||
| + | [[Category: Nikonov, S]] | ||
| + | [[Category: Stolboushkina, E]] | ||
| + | [[Category: Aif2]] | ||
| + | [[Category: Aif2gamma]] | ||
| + | [[Category: Aif2gamma switch]] | ||
| + | [[Category: Conformational change]] | ||
| + | [[Category: G-protein]] | ||
| + | [[Category: Gtp hydrolysis]] | ||
| + | [[Category: Gtp-binding domain]] | ||
| + | [[Category: Magnesium ion]] | ||
| + | [[Category: Translation]] | ||
| + | [[Category: Translation gtpase]] | ||
| + | [[Category: Translation initiation]] | ||
Current revision
The structure of the FKR mutant of the archaeal translation initiation factor 2 gamma subunit in complex with GDPCP, obtained in the absence of magnesium salts in the crystallization solution.
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Categories: Large Structures | Sacs2 | Gabdulkhakov, A | Garber, M | Kravchenko, O | Nevskaya, N | Nikonov, O | Nikonov, S | Stolboushkina, E | Aif2 | Aif2gamma | Aif2gamma switch | Conformational change | G-protein | Gtp hydrolysis | Gtp-binding domain | Magnesium ion | Translation | Translation gtpase | Translation initiation
