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2opa

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[[Image:2opa.jpg|left|200px]]
 
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{{Structure
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==YwhB binary complex with 2-Fluoro-p-hydroxycinnamate==
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|PDB= 2opa |SIZE=350|CAPTION= <scene name='initialview01'>2opa</scene>, resolution 2.4&Aring;
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<StructureSection load='2opa' size='340' side='right'caption='[[2opa]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Fhc+Binding+Site+For+Residue+A+500'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FHC:2-FLUORO-3-(4-HYDROXYPHENYL)-2E-PROPENEOATE'>FHC</scene>
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<table><tr><td colspan='2'>[[2opa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OPA FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= ywhB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FHC:2-FLUORO-3-(4-HYDROXYPHENYL)-2E-PROPENEOATE'>FHC</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2opa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opa OCA], [https://pdbe.org/2opa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2opa RCSB], [https://www.ebi.ac.uk/pdbsum/2opa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2opa ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2op8|2OP8]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2opa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opa OCA], [http://www.ebi.ac.uk/pdbsum/2opa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2opa RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/4OT_BACSU 4OT_BACSU] Catalyzes both 1,3- and 1,5-keto-enol tautomerization of the diacid 2-hydroxymuconate (2-hydroxy-2,4-hexadienedioate) to produce 2-oxo-4-hexenedioate. This reaction is highly stereoselective and produces a mixture of stereoisomers, where the (3S)-isomer of 2-oxo-4-hexenedioate predominates. Also catalyzes the tautomerization of 2-hydroxymuconate to 2-oxo-3-hexenedioate, however this reaction is slower and occurs after the tautomerization of 2-hydroxymuconate to 2-oxo-4-hexenedioate. Using 2-hydroxy-2,4-pentadienoate, phenylenolpyruvate, (p-hydroxyphenyl)-enolpyruvate and 2-hydroxy-2,4-heptadiene-1,7-dioate, YwhB is a highly efficient 1,3-keto-enol tautomerase, but clearly not a 1,5-keto-enol tautomerase. Tautomerization of the two monoacids 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate produces a mixture of stereoisomers, where the (3R)-isomers predominate.<ref>PMID:17902707</ref>
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== Evolutionary Conservation ==
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'''YwhB binary complex with 2-Fluoro-p-hydroxycinnamate'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/2opa_consurf.spt"</scriptWhenChecked>
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2OPA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPA OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2opa ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Almrud, J J.]]
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[[Category: Almrud JJ]]
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[[Category: Hackert, M L.]]
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[[Category: Hackert ML]]
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[[Category: Whitman, C P.]]
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[[Category: Whitman CP]]
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[[Category: 2-fluoro-p-hydroxycinnamate]]
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[[Category: 4-oxalocrotonate tautomerase]]
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[[Category: homohexamer]]
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[[Category: inhibitor]]
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[[Category: isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:09 2008''
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Current revision

YwhB binary complex with 2-Fluoro-p-hydroxycinnamate

PDB ID 2opa

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