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6g5s

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Solution structure of the TPR domain of the cell division coordinator, CpoB

Structural highlights

6g5s is a 1 chain structure with sequence from Escherichia coli K-12. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPOB_ECOLI Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.^[1]

Publication Abstract from PubMed

Bacteria surround their cytoplasmic membrane with an essential, stress-bearing peptidoglycan (PG) layer consisting of glycan chains linked by short peptides into a mesh-like structure. Growing and dividing cells expand their PG layer using inner-membrane anchored PG synthases, including Penicillin-binding proteins (PBPs), which participate in dynamic protein complexes to facilitate cell wall growth. In Escherichia coli, and presumably other Gram-negative bacteria, growth of the mainly single layered PG is regulated by outer membrane-anchored lipoproteins. The lipoprotein LpoB is required to activate PBP1B, which is a major, bi-functional PG synthase with glycan chain polymerizing (glycosyltransferase) and peptide cross-linking (transpeptidase) activities. In this work we show how the binding of LpoB to the regulatory UB2H domain of PBP1B activates both activities. Binding induces structural changes in the UB2H domain, which transduce to the two catalytic domains by distinct allosteric pathways. We also show how an additional regulator protein, CpoB, is able to selectively modulate the TPase activation by LpoB without interfering with GTase activation. This article is protected by copyright. All rights reserved.

Induced conformational changes activate the peptidoglycan synthase PBP1B.,Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP Mol Microbiol. 2018 Jul 25. doi: 10.1111/mmi.14082. PMID:30044025^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar AF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W. Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. Elife. 2015 May 7;4. doi: 10.7554/eLife.07118. PMID:25951518 doi:http://dx.doi.org/10.7554/eLife.07118
↑ Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP. Induced conformational changes activate the peptidoglycan synthase PBP1B. Mol Microbiol. 2018 Jul 25. doi: 10.1111/mmi.14082. PMID:30044025 doi:http://dx.doi.org/10.1111/mmi.14082

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6g5s"

@@ Line 1: / Line 1: @@
 ==Solution structure of the TPR domain of the cell division coordinator, CpoB==
-<StructureSection load='6g5s' size='340' side='right' caption='[[6g5s]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='6g5s' size='340' side='right'caption='[[6g5s]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6g5s]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G5S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6g5s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G5S FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xev|2xev]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g5s OCA], [https://pdbe.org/6g5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g5s RCSB], [https://www.ebi.ac.uk/pdbsum/6g5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g5s ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g5s OCA], [http://pdbe.org/6g5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g5s RCSB], [http://www.ebi.ac.uk/pdbsum/6g5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g5s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CPOB_ECOLI CPOB_ECOLI]] Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.<ref>PMID:25951518</ref>
+[https://www.uniprot.org/uniprot/CPOB_ECOLI CPOB_ECOLI] Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.<ref>PMID:25951518</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Bougault, C]]
+[[Category: Escherichia coli K-12]]
-[[Category: Egan, A]]
+[[Category: Large Structures]]
-[[Category: Martinez, R C.Maya]]
+[[Category: Bougault C]]
-[[Category: Simorre, J P]]
+[[Category: Egan A]]
-[[Category: Vollmer, W]]
+[[Category: Maya Martinez RC]]
-[[Category: Cell cycle]]
+[[Category: Simorre JP]]
-[[Category: Cell division coordinator]]
+[[Category: Vollmer W]]
-[[Category: E. coli]]
-[[Category: Pbp1b]]
-[[Category: Transpeptidase regulator]]

6g5s

From Proteopedia

Current revision

Solution structure of the TPR domain of the cell division coordinator, CpoB

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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