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| - | [[Image:2ost.gif|left|200px]] | |
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| - | {{Structure
| + | ==The structure of a bacterial homing endonuclease : I-Ssp6803I== |
| - | |PDB= 2ost |SIZE=350|CAPTION= <scene name='initialview01'>2ost</scene>, resolution 3.100Å
| + | <StructureSection load='2ost' size='340' side='right'caption='[[2ost]], [[Resolution|resolution]] 3.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OST FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [https://pdbe.org/2ost PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [https://www.ebi.ac.uk/pdbsum/2ost PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ost ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [http://www.ebi.ac.uk/pdbsum/2ost PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q57253_SYNY3 Q57253_SYNY3] |
| - | | + | __TOC__ |
| - | '''The structure of a bacterial homing endonuclease : I-Ssp6803I'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Synechocystis sp. PCC 6803]] |
| - | ==Overview==
| + | [[Category: Bonocora RP]] |
| - | The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
| + | [[Category: Shub DA]] |
| - | | + | [[Category: Stoddard BL]] |
| - | ==About this Structure==
| + | [[Category: Zhao L]] |
| - | 2OST is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif., Zhao L, Bonocora RP, Shub DA, Stoddard BL, EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17410205 17410205]
| + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Synechocystis sp.]] | + | |
| - | [[Category: Bonocora, R P.]] | + | |
| - | [[Category: Shub, D A.]] | + | |
| - | [[Category: Stoddard, B L.]] | + | |
| - | [[Category: Zhao, L.]] | + | |
| - | [[Category: group i intron]]
| + | |
| - | [[Category: homing endonuclease]]
| + | |
| - | [[Category: protein-dna complex]]
| + | |
| - | [[Category: restriction enzyme fold,pd-(d/e)-xk motif]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:22:35 2008''
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