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| ==Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC== | | ==Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC== |
- | <StructureSection load='2m8u' size='340' side='right' caption='[[2m8u]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2m8u' size='340' side='right'caption='[[2m8u]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[2m8u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M8U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2m8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M8U FirstGlance]. <br> |
- | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DDB_G0289563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8u OCA], [http://pdbe.org/2m8u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m8u RCSB], [http://www.ebi.ac.uk/pdbsum/2m8u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m8u ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8u OCA], [https://pdbe.org/2m8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m8u RCSB], [https://www.ebi.ac.uk/pdbsum/2m8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m8u ProSAT]</span></td></tr> |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/Q54HC2_DICDI Q54HC2_DICDI] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Atcc 11735]] | + | [[Category: Dictyostelium discoideum]] |
- | [[Category: Chitayat, S]] | + | [[Category: Large Structures]] |
- | [[Category: Cote, G P]] | + | [[Category: Chitayat S]] |
- | [[Category: Crawley, S W]] | + | [[Category: Cote GP]] |
- | [[Category: Langelaan, D]] | + | [[Category: Crawley SW]] |
- | [[Category: Liburd, J D]] | + | [[Category: Langelaan D]] |
- | [[Category: Miller, E]] | + | [[Category: Liburd JD]] |
- | [[Category: Smith, S P]] | + | [[Category: Miller E]] |
- | [[Category: Contractile protein]]
| + | [[Category: Smith SP]] |
- | [[Category: Helical bundle]]
| + | |
- | [[Category: Myosin light chain]]
| + | |
| Structural highlights
Function
Q54HC2_DICDI
Publication Abstract from PubMed
Myosin light chains are key regulators of class 1 myosins and typically comprise two domains, with calmodulin being the archetypal example. They bind IQ motifs within the myosin neck region and amplify conformational changes in motor domain. A single lobe light chain, myosin light chain C (MlcC), was recently identified and shown to specifically bind to two sequentially divergent IQ motifs of the Dictyostelium myosin-1C. Towards providing a molecular basis of this interaction, the structures of apo-MlcC and a 2:1 MlcC:myosin-1C neck complex were determined. The two non-functional EF-hand motifs of MlcC pack together to form a globular four-helix bundle that opens up to expose a central hydrophobic groove, which interacts the N-terminal portion of the divergent IQ1 and IQ2 motifs. N- and C-terminal regions of MlcC make critical contacts that contribute to its specific interactions with the myosin-1C divergent IQ motifs; contacts that deviate from the traditional mode of calmodulin-IQ recognition.
Structure of the single-lobe myosin light chain C in complex with the light chain-binding domains of myosin-1C provides insights into divergent IQ-motif recognition.,Langelaan DN, Liburd J, Yang Y, Miller E, Chitayat S, Crawley SW, Cote GP, Smith SP J Biol Chem. 2016 Jul 27. pii: jbc.M116.746313. PMID:27466369[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Langelaan DN, Liburd J, Yang Y, Miller E, Chitayat S, Crawley SW, Cote GP, Smith SP. Structure of the single-lobe myosin light chain C in complex with the light chain-binding domains of myosin-1C provides insights into divergent IQ-motif recognition. J Biol Chem. 2016 Jul 27. pii: jbc.M116.746313. PMID:27466369 doi:http://dx.doi.org/10.1074/jbc.M116.746313
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