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| - | {{Large structure}}
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| | ==Serine 16 phosphorylated phospholamban pentamer, Hybrid solution and solid-state NMR structural ensemble== | | ==Serine 16 phosphorylated phospholamban pentamer, Hybrid solution and solid-state NMR structural ensemble== |
| - | <StructureSection load='2m3b' size='340' side='right' caption='[[2m3b]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2m3b' size='340' side='right'caption='[[2m3b]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2m3b]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M3B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2m3b]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M3B FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solid-state NMR , Solution NMR, 20 models</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kyv|2kyv]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PLN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3b OCA], [https://pdbe.org/2m3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3b RCSB], [https://www.ebi.ac.uk/pdbsum/2m3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3b ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3b OCA], [http://pdbe.org/2m3b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m3b RCSB], [http://www.ebi.ac.uk/pdbsum/2m3b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3b ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | {{Large structure}} | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PPLA_RABIT PPLA_RABIT]] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. | + | [https://www.uniprot.org/uniprot/PPLA_RABIT PPLA_RABIT] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European rabbit]] | + | [[Category: Large Structures]] |
| - | [[Category: Veglia, G]] | + | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Verardi, R]] | + | [[Category: Veglia G]] |
| - | [[Category: Vostrikov, V V]] | + | [[Category: Verardi R]] |
| - | [[Category: Membrane protein]] | + | [[Category: Vostrikov VV]] |
| - | [[Category: Phospholamban]]
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| - | [[Category: Plb]]
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| - | [[Category: Pln]]
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| Structural highlights
Function
PPLA_RABIT Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
Publication Abstract from PubMed
Phospholamban (PLN) inhibits the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA), thereby regulating cardiac diastole. In membranes, PLN assembles into homopentamers that in both the phosphorylated and nonphosphorylated states have been proposed to form ion-selective channels. Here, we determined the structure of the phosphorylated pentamer using a combination of solution and solid-state nuclear magnetic resonance methods. We found that the pinwheel architecture of the homopentamer is preserved upon phosphorylation, with each monomer having an L-shaped conformation. The TM domains form a hydrophobic pore approximately 24 A long and 2 A in diameter, which is inconsistent with canonical Ca2+-selective channels. Phosphorylation, however, enhances the conformational dynamics of the cytoplasmic region of PLN, causing partial unwinding of the amphipathic helix. We propose that PLN oligomers act as storage for active monomers, keeping SERCA function within a physiological window.
Structural Dynamics and Topology of Phosphorylated Phospholamban Homopentamer Reveal Its Role in the Regulation of Calcium Transport.,Vostrikov VV, Mote KR, Verardi R, Veglia G Structure. 2013 Oct 22. pii: S0969-2126(13)00358-4. doi:, 10.1016/j.str.2013.09.008. PMID:24207128[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Vostrikov VV, Mote KR, Verardi R, Veglia G. Structural Dynamics and Topology of Phosphorylated Phospholamban Homopentamer Reveal Its Role in the Regulation of Calcium Transport. Structure. 2013 Oct 22. pii: S0969-2126(13)00358-4. doi:, 10.1016/j.str.2013.09.008. PMID:24207128 doi:http://dx.doi.org/10.1016/j.str.2013.09.008
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