|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity== | | ==NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity== |
| - | <StructureSection load='2lus' size='340' side='right' caption='[[2lus]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lus' size='340' side='right'caption='[[2lus]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lus]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carro Carro]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LUS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lus]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carcinoscorpius_rotundicauda Carcinoscorpius rotundicauda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lus OCA], [http://pdbe.org/2lus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lus RCSB], [http://www.ebi.ac.uk/pdbsum/2lus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lus ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lus OCA], [https://pdbe.org/2lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lus RCSB], [https://www.ebi.ac.uk/pdbsum/2lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lus ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A3E0V9_CARRO A3E0V9_CARRO] |
| - | Thioredoxins (Trxs), which play a key role in maintaining a redox environment in the cell, are found in almost all organisms. Trxs act as potential reducing agents of disulfide bonds and contain two vicinal cysteines in a CXXC motif at the active site. Trx is also known to activate the DNA binding activity of NF-kappaB, an important transcription factor. Previously, Trx-related protein 16 from Carcinoscorpius rotundicauda (Cr-TRP16), a 16-kDa Trx-like protein that contains a WCPPC motif, was reported. Here we present the NMR structure of the reduced form of Cr-TRP16, along with its regulation of NF-kappaB activity. Unlike other 16-kDa Trx-like proteins, Cr-TRP16 contains an additional Cys residue (Cys-15, at the N terminus), through which it forms a homodimer. Moreover, we have explored the molecular basis of Cr-TRP16-mediated activation of NF-kappaB and showed that Cr-TRP16 exists as a dimer under physiological conditions, and only the dimeric form binds to NF-kappaB and enhances its DNA binding activity by directly reducing the cysteines in the DNA-binding motif of NF-kappaB. The C15S mutant of Cr-TRP16 was unable to dimerize and hence does not bind to NF-kappaB. Based on our finding and combined with the literature, we propose a model of how Cr-TRP16 is likely to bind to NF-kappaB. These findings elucidate the molecular mechanism by which NF-kappaB activation is regulated through Cr-TRP16.
| + | |
| - | | + | |
| - | NMR structure of Carcinoscorpius rotundicauda thioredoxin-related protein 16 and its role in regulating transcription factor NF-kappaB activity.,Giri PK, Jing-Song F, Shanmugam MK, Ding JL, Sethi G, Swaminathan K, Sivaraman J J Biol Chem. 2012 Aug 24;287(35):29417-28. doi: 10.1074/jbc.M112.379859. Epub, 2012 Jul 3. PMID:22763700<ref>PMID:22763700</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 2lus" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Carro]] | + | [[Category: Carcinoscorpius rotundicauda]] |
| - | [[Category: Fan, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Giri, P K]] | + | [[Category: Fan J]] |
| - | [[Category: Kunchithapadam, S]] | + | [[Category: Giri PK]] |
| - | [[Category: Sivaraman, J]] | + | [[Category: Kunchithapadam S]] |
| - | [[Category: Cr-trp16]]
| + | [[Category: Sivaraman J]] |
| - | [[Category: Oxidoreductase]]
| + | |
