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| | ==NMR structure of Sp140 PHD finger cis conformer== | | ==NMR structure of Sp140 PHD finger cis conformer== |
| - | <StructureSection load='2md8' size='340' side='right' caption='[[2md8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2md8' size='340' side='right'caption='[[2md8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2md8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MD8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MD8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2md8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MD8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2md7|2md7]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2md8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2md8 OCA], [http://pdbe.org/2md8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2md8 RCSB], [http://www.ebi.ac.uk/pdbsum/2md8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2md8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2md8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2md8 OCA], [https://pdbe.org/2md8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2md8 RCSB], [https://www.ebi.ac.uk/pdbsum/2md8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2md8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | == Function == | |
| - | [[http://www.uniprot.org/uniprot/SP140_HUMAN SP140_HUMAN]] Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. | |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Musco, G]] | + | [[Category: Large Structures]] |
| - | [[Category: Quilici, G]] | + | [[Category: Musco G]] |
| - | [[Category: Zucchelli, C]] | + | [[Category: Quilici G]] |
| - | [[Category: Phd finger]] | + | [[Category: Zucchelli C]] |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Sp140 is a nuclear leukocyte-specific protein involved in primary biliary cirrhosis and a risk factor in chronic lymphocytic leukemia. The presence of several chromatin related modules such as plant homeodomain (PHD), bromodomain and SAND domain suggests a role in chromatin-mediated regulation of gene expression; however, its real function is still elusive. Herein we present the solution structure of Sp140-PHD finger and investigate its role as epigenetic reader in vitro. Sp140-PHD presents an atypical PHD finger fold which does not bind to histone H3 tails but is recognized by peptidylprolyl isomerase Pin1. Pin1 specifically binds to a phosphopeptide corresponding to the L3 loop of Sp140-PHD and catalyzes cis-trans isomerization of a p Thr-Pro bond. Moreover co-immunoprecipitation experiments demonstrate FLAG-Sp140 interaction with endogenous Pin1 in vivo. Overall these data include Sp140 in the list of the increasing number of Pin1 binders and expand the regulatory potential of PHD fingers as versatile structural platforms for diversified interactions.
Structure of human Sp140 PHD finger: an atypical fold interacting with Pin1.,Zucchelli C, Tamburri S, Quilici G, Palagano E, Berardi A, Saare M, Peterson P, Bachi A, Musco G FEBS J. 2014 Jan;281(1):216-31. doi: 10.1111/febs.12588. Epub 2013 Nov 25. PMID:24267382[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zucchelli C, Tamburri S, Quilici G, Palagano E, Berardi A, Saare M, Peterson P, Bachi A, Musco G. Structure of human Sp140 PHD finger: an atypical fold interacting with Pin1. FEBS J. 2014 Jan;281(1):216-31. doi: 10.1111/febs.12588. Epub 2013 Nov 25. PMID:24267382 doi:http://dx.doi.org/10.1111/febs.12588
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