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| | ==Solution structure of the Brazil Nut 2S albumin Ber e 1== | | ==Solution structure of the Brazil Nut 2S albumin Ber e 1== |
| - | <StructureSection load='2lvf' size='340' side='right' caption='[[2lvf]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''> | + | <StructureSection load='2lvf' size='340' side='right'caption='[[2lvf]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Berex Berex]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LVF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bertholletia_excelsa Bertholletia excelsa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvf OCA], [http://pdbe.org/2lvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lvf RCSB], [http://www.ebi.ac.uk/pdbsum/2lvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvf ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 12 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvf OCA], [https://pdbe.org/2lvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvf RCSB], [https://www.ebi.ac.uk/pdbsum/2lvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/2SS1_BEREX 2SS1_BEREX] This is a 2S seed storage protein. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Albumin|Albumin]] | + | *[[Albumin 3D structures|Albumin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Berex]] | + | [[Category: Bertholletia excelsa]] |
| - | [[Category: Alcocer, M J]] | + | [[Category: Large Structures]] |
| - | [[Category: Larsson, G]] | + | [[Category: Alcocer MJ]] |
| - | [[Category: Rundqvist, L]] | + | [[Category: Larsson G]] |
| - | [[Category: Schleucher, J]] | + | [[Category: Rundqvist L]] |
| - | [[Category: Tengel, T]] | + | [[Category: Schleucher J]] |
| - | [[Category: Zdunek, J]] | + | [[Category: Tengel T]] |
| - | [[Category: Allergen]]
| + | [[Category: Zdunek J]] |
| - | [[Category: Copper binding]]
| + | |
| - | [[Category: Hydrophobic interaction]]
| + | |
| Structural highlights
Function
2SS1_BEREX This is a 2S seed storage protein.
Publication Abstract from PubMed
BACKGROUND: The 2S albumin Ber e 1 is the major allergen in Brazil nuts. Previous findings indicated that the protein alone does not cause an allergenic response in mice, but the addition of components from a Brazil nut lipid fraction were required. Structural details of Ber e 1 may contribute to the understanding of the allergenic properties of the protein and its potential interaction partners. METHODOLOGY/PRINCIPAL FINDINGS: The solution structure of recombinant Ber e 1 was solved using NMR spectroscopy and measurements of the protein back bone dynamics at a residue-specific level were extracted using (15)N-spin relaxation. A hydrophobic cavity was identified in the structure of Ber e 1. Using the paramagnetic relaxation enhancement property of Cu(2+) in conjunction with NMR, it was shown that Ber e 1 is able to specifically interact with the divalent copper ion and the binding site was modeled into the structure. The IgE binding region as well as the copper binding site show increased dynamics on both fast ps-ns timescale as well as slower micros-ms timescale. CONCLUSIONS/SIGNIFICANCE: The overall fold of Ber e 1 is similar to other 2S albumins, but the hydrophobic cavity resembles that of a homologous non-specific lipid transfer protein. Ber e 1 is the first 2S albumin shown to interact with Cu(2+) ions. This Cu(2+) binding has minimal effect on the electrostatic potential on the surface of the protein, but the charge distribution within the hydrophobic cavity is significantly altered. As the hydrophobic cavity is likely to be involved in a putative lipid interaction the Cu(2+) can in turn affect the interaction that is essential to provoke an allergenic response.
Solution structure, copper binding and backbone dynamics of recombinant Ber e 1-the major allergen from Brazil nut.,Rundqvist L, Tengel T, Zdunek J, Bjorn E, Schleucher J, Alcocer MJ, Larsson G PLoS One. 2012;7(10):e46435. doi: 10.1371/journal.pone.0046435. Epub 2012 Oct 4. PMID:23056307[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rundqvist L, Tengel T, Zdunek J, Bjorn E, Schleucher J, Alcocer MJ, Larsson G. Solution structure, copper binding and backbone dynamics of recombinant Ber e 1-the major allergen from Brazil nut. PLoS One. 2012;7(10):e46435. doi: 10.1371/journal.pone.0046435. Epub 2012 Oct 4. PMID:23056307 doi:http://dx.doi.org/10.1371/journal.pone.0046435
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