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| | ==NMR structure of E. coli LpoB== | | ==NMR structure of E. coli LpoB== |
| - | <StructureSection load='2mii' size='340' side='right' caption='[[2mii]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mii' size='340' side='right'caption='[[2mii]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mii]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecodh Ecodh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MII FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mii]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._DH10B Escherichia coli str. K-12 substr. DH10B]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MII FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ycfM, lpoB, ECDH10B_1177 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316385 ECODH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mii OCA], [http://pdbe.org/2mii PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mii RCSB], [http://www.ebi.ac.uk/pdbsum/2mii PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mii ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mii OCA], [https://pdbe.org/2mii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mii RCSB], [https://www.ebi.ac.uk/pdbsum/2mii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mii ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | == Function == | |
| - | [[http://www.uniprot.org/uniprot/B1XA15_ECODH B1XA15_ECODH]] Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1B (PBP1b) (By similarity).[HAMAP-Rule:MF_01889] | |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecodh]] | + | [[Category: Escherichia coli str. K-12 substr. DH10B]] |
| - | [[Category: Bougault, C M]] | + | [[Category: Large Structures]] |
| - | [[Category: Egan, A J.F]] | + | [[Category: Bougault CM]] |
| - | [[Category: Jean, N L]] | + | [[Category: Egan AJF]] |
| - | [[Category: Koumoutsi, A]] | + | [[Category: Jean NL]] |
| - | [[Category: Simorre, J P]] | + | [[Category: Koumoutsi A]] |
| - | [[Category: Typas, A]] | + | [[Category: Simorre JP]] |
| - | [[Category: Vollmer, W]] | + | [[Category: Typas A]] |
| - | [[Category: Lpob]]
| + | [[Category: Vollmer W]] |
| - | [[Category: Pbp1b activator]]
| + | |
| - | [[Category: Peptidoglycan synthesis]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Bacteria surround their cytoplasmic membrane with an essential, stress-bearing peptidoglycan (PG) layer. Growing and dividing cells expand their PG layer by using membrane-anchored PG synthases, which are guided by dynamic cytoskeletal elements. In Escherichia coli, growth of the mainly single-layered PG is also regulated by outer membrane-anchored lipoproteins. The lipoprotein LpoB is required for the activation of penicillin-binding protein (PBP) 1B, which is a major, bifunctional PG synthase with glycan chain polymerizing (glycosyltransferase) and peptide cross-linking (transpeptidase) activities. Here, we report the structure of LpoB, determined by NMR spectroscopy, showing an N-terminal, 54-aa-long flexible stretch followed by a globular domain with similarity to the N-terminal domain of the prevalent periplasmic protein TolB. We have identified the interaction interface between the globular domain of LpoB and the noncatalytic UvrB domain 2 homolog domain of PBP1B and modeled the complex. Amino acid exchanges within this interface weaken the PBP1B-LpoB interaction, decrease the PBP1B stimulation in vitro, and impair its function in vivo. On the contrary, the N-terminal flexible stretch of LpoB is required to stimulate PBP1B in vivo, but is dispensable in vitro. This supports a model in which LpoB spans the periplasm to interact with PBP1B and stimulate PG synthesis.
Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B.,Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8197-202. doi:, 10.1073/pnas.1400376111. Epub 2014 May 12. PMID:24821816[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP. Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8197-202. doi:, 10.1073/pnas.1400376111. Epub 2014 May 12. PMID:24821816 doi:http://dx.doi.org/10.1073/pnas.1400376111
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