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| - | [[Image:2ow8.gif|left|200px]]
| + | #REDIRECT [[4v4i]] This PDB entry is obsolete and replaced by 4v4i |
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| - | {{Structure
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| - | |PDB= 2ow8 |SIZE=350|CAPTION= <scene name='initialview01'>2ow8</scene>, resolution 3.710Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5'-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2'-METHYLCYTIDINE-5'-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=4SU:4-THIOURIDINE-5'-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5'-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5'-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5'-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5'-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5'-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5'-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5'-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5'-MONOPHOSHATE'>UR3</scene>
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| - | |ACTIVITY=
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| - | |GENE=
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| - | |DOMAIN=
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| - | |RELATEDENTRY=[[1gix|1GIX]], [[1giy|1GIY]], [[1vsa|1VSA]]
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ow8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ow8 OCA], [http://www.ebi.ac.uk/pdbsum/2ow8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ow8 RCSB]</span>
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| - | }}
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| - | '''Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. This file, 2OW8, contains the 30S ribosome subunit, two tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1VSA.'''
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| - | ==Overview==
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| - | Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal structure of the Thermus thermophilus 70S ribosome containing a model mRNA and two tRNAs at 3.7 A resolution. Many structural details of the interactions between the ribosome, tRNA, and mRNA in the P and E sites and the ways in which tRNA structure is distorted by its interactions with the ribosome are seen. Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site.
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| - | ==About this Structure==
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| - | 2OW8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entry 2I1C. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OW8 OCA].
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| - | ==Reference==
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| - | Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements., Korostelev A, Trakhanov S, Laurberg M, Noller HF, Cell. 2006 Sep 22;126(6):1065-77. Epub 2006 Sep 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16962654 16962654]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Korostelev, A.]]
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| - | [[Category: Laurberg, M.]]
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| - | [[Category: Noller, H F.]]
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| - | [[Category: Trakhanov, S.]]
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| - | [[Category: crystal structure]]
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| - | [[Category: e site]]
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| - | [[Category: functional complex]]
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| - | [[Category: p site]]
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| - | [[Category: peptidyl transferase center]]
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| - | [[Category: ribosome]]
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| - | [[Category: trna]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:24:03 2008''
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