2ozb

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[[Image:2ozb.gif|left|200px]]
 
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{{Structure
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==Structure of a human Prp31-15.5K-U4 snRNA complex==
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|PDB= 2ozb |SIZE=350|CAPTION= <scene name='initialview01'>2ozb</scene>, resolution 2.60&Aring;
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<StructureSection load='2ozb' size='340' side='right'caption='[[2ozb]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[2ozb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OZB FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE= NHP2L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PRPF31, PRP31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ozb OCA], [https://pdbe.org/2ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ozb RCSB], [https://www.ebi.ac.uk/pdbsum/2ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ozb ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1e7k|1E7K]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ozb OCA], [http://www.ebi.ac.uk/pdbsum/2ozb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ozb RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/2ozb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ozb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Although highly homologous, the spliceosomal hPrp31 and the nucleolar Nop56 and Nop58 (Nop56/58) proteins recognize different ribonucleoprotein (RNP) particles. hPrp31 interacts with complexes containing the 15.5K protein and U4 or U4atac small nuclear RNA (snRNA), whereas Nop56/58 associate with 15.5K-box C/D small nucleolar RNA complexes. We present structural and biochemical analyses of hPrp31-15.5K-U4 snRNA complexes that show how the conserved Nop domain in hPrp31 maintains high RNP binding selectivity despite relaxed RNA sequence requirements. The Nop domain is a genuine RNP binding module, exhibiting RNA and protein binding surfaces. Yeast two-hybrid analyses suggest a link between retinitis pigmentosa and an aberrant hPrp31-hPrp6 interaction that blocks U4/U6-U5 tri-snRNP formation.
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'''Structure of a human Prp31-15.5K-U4 snRNA complex'''
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Binding of the human Prp31 Nop domain to a composite RNA-protein platform in U4 snRNP.,Liu S, Li P, Dybkov O, Nottrott S, Hartmuth K, Luhrmann R, Carlomagno T, Wahl MC Science. 2007 Apr 6;316(5821):115-20. PMID:17412961<ref>PMID:17412961</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ozb" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2OZB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZB OCA].
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Liu, S.]]
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[[Category: Liu S]]
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[[Category: Luehrmann, R.]]
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[[Category: Luehrmann R]]
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[[Category: Wahl, M C.]]
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[[Category: Wahl MC]]
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[[Category: hierarchical assembly]]
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[[Category: nop domain]]
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[[Category: pre-mrna splicing]]
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[[Category: ribonucleoprotein particle (rnp)]]
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[[Category: rna binding protein/rna complex]]
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[[Category: rna-protein complex]]
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[[Category: rnp-binding domain]]
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[[Category: u4/u6 di-snrna]]
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[[Category: u4/u6 di-snrnp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:25:24 2008''
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Current revision

Structure of a human Prp31-15.5K-U4 snRNA complex

PDB ID 2ozb

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