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| | ==NMR structure of the RRM3 domain of Gbp2== | | ==NMR structure of the RRM3 domain of Gbp2== |
| - | <StructureSection load='2mzq' size='340' side='right' caption='[[2mzq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mzq' size='340' side='right'caption='[[2mzq]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MZQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mzq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MZQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2mzr|2mzr]], [[2mzs|2mzs]], [[2mzt|2mzt]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GBP2, RLF6, YCL011C, YCL11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mzq OCA], [https://pdbe.org/2mzq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mzq RCSB], [https://www.ebi.ac.uk/pdbsum/2mzq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mzq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mzq OCA], [http://pdbe.org/2mzq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mzq RCSB], [http://www.ebi.ac.uk/pdbsum/2mzq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mzq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GBP2_YEAST GBP2_YEAST]] Binds single-stranded telomeric sequences of the type (TG[1-3])n in vitro. Also binds to RNA. Influences the localization of RAP1 in the nuclei. Involved in modulating telomere length. | + | [https://www.uniprot.org/uniprot/GBP2_YEAST GBP2_YEAST] Binds single-stranded telomeric sequences of the type (TG[1-3])n in vitro. Also binds to RNA. Influences the localization of RAP1 in the nuclei. Involved in modulating telomere length. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Martinez-Lumbreras, S]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Perez-Canadillas, J]] | + | [[Category: Martinez-Lumbreras S]] |
| - | [[Category: Seraphin, B]] | + | [[Category: Perez-Canadillas J]] |
| - | [[Category: Gbp2]]
| + | [[Category: Seraphin B]] |
| - | [[Category: Rna binding domain]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rrm]]
| + | |
| - | [[Category: Tho/trex]]
| + | |
| Structural highlights
Function
GBP2_YEAST Binds single-stranded telomeric sequences of the type (TG[1-3])n in vitro. Also binds to RNA. Influences the localization of RAP1 in the nuclei. Involved in modulating telomere length.
Publication Abstract from PubMed
Metazoan SR and SR-like proteins are important regulatory factors in RNA splicing, export, translation and RNA decay. We determined the NMR structures and nucleic acid interaction modes of Gbp2 and Hrb1, two paralogous budding yeast proteins with similarities to mammalian SR proteins. Gbp2 RRM1 and RRM2 recognise preferentially RNAs containing the core motif GGUG. Sequence selectivity resides in a non-canonical interface in RRM2 that is highly related to the SRSF1 pseudoRRM. The atypical Gbp2/Hrb1 C-terminal RRM domains (RRM3) do not interact with RNA/DNA, likely because of their novel N-terminal extensions that block the canonical RNA binding interface. Instead, we discovered that RRM3 is crucial for interaction with the THO/TREX complex and identified key residues essential for this interaction. Moreover, Gbp2 interacts genetically with Tho2 as the double deletion shows a synthetic phenotype and preventing Gbp2 interaction with the THO/TREX complex partly supresses gene expression defect associated with inactivation of the latter complex. These findings provide structural and functional insights into the contribution of SR-like proteins in the post-transcriptional control of gene expression.
Gbp2 interacts with THO/TREX through a novel type of RRM domain.,Martinez-Lumbreras S, Taverniti V, Zorrilla S, Seraphin B, Perez-Canadillas JM Nucleic Acids Res. 2015 Nov 23. pii: gkv1303. PMID:26602689[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Martinez-Lumbreras S, Taverniti V, Zorrilla S, Seraphin B, Perez-Canadillas JM. Gbp2 interacts with THO/TREX through a novel type of RRM domain. Nucleic Acids Res. 2015 Nov 23. pii: gkv1303. PMID:26602689 doi:http://dx.doi.org/10.1093/nar/gkv1303
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