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| | ==NMR Solution Structure of Aureocin A53== | | ==NMR Solution Structure of Aureocin A53== |
| - | <StructureSection load='2n8o' size='340' side='right' caption='[[2n8o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n8o' size='340' side='right'caption='[[2n8o]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n8o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n8o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8O FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2n8p|2n8p]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aucA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8o OCA], [https://pdbe.org/2n8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8o RCSB], [https://www.ebi.ac.uk/pdbsum/2n8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8o ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8o OCA], [http://pdbe.org/2n8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8o RCSB], [http://www.ebi.ac.uk/pdbsum/2n8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/AUREO_STAAU AUREO_STAAU] |
| - | Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four alpha-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 A) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against the Gram-positive strains tested, suggesting that sequence disparities play a crucial role in their mechanisms of action.
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| - | Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.,Acedo JZ, van Belkum MJ, Lohans CT, Towle KM, Miskolzie M, Vederas JC Biochemistry. 2016 Feb 2;55(4):733-42. doi: 10.1021/acs.biochem.5b01306. Epub, 2016 Jan 15. PMID:26771761<ref>PMID:26771761</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 2n8o" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acedo, J Z]] | + | [[Category: Large Structures]] |
| - | [[Category: Belkum, M J.van]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Lohans, C T]] | + | [[Category: Acedo JZ]] |
| - | [[Category: Miskolzie, M]] | + | [[Category: Lohans CT]] |
| - | [[Category: Towle, K M]] | + | [[Category: Miskolzie M]] |
| - | [[Category: Vederas, J C]] | + | [[Category: Towle KM]] |
| - | [[Category: Antimicrobial protein]] | + | [[Category: Vederas JC]] |
| - | [[Category: Bacteriocin]] | + | [[Category: Van Belkum MJ]] |
| - | [[Category: Leaderless bacteriocin]]
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