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| | ==Structure of the Geobacillus stearothermophilus IF2 G3-subdomain== | | ==Structure of the Geobacillus stearothermophilus IF2 G3-subdomain== |
| - | <StructureSection load='2nbg' size='340' side='right' caption='[[2nbg]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2nbg' size='340' side='right'caption='[[2nbg]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2nbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NBG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2nbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NBG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">infB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nbg OCA], [https://pdbe.org/2nbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nbg RCSB], [https://www.ebi.ac.uk/pdbsum/2nbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nbg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nbg OCA], [http://pdbe.org/2nbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nbg RCSB], [http://www.ebi.ac.uk/pdbsum/2nbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nbg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE]] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. | + | [https://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 12980]] | + | [[Category: Geobacillus stearothermophilus]] |
| - | [[Category: Boelens, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Dongre, R]] | + | [[Category: Boelens R]] |
| - | [[Category: Folkers, G E]] | + | [[Category: Dongre R]] |
| - | [[Category: Gualerzi, C O]] | + | [[Category: Folkers GE]] |
| - | [[Category: Wienk, H]] | + | [[Category: Gualerzi CO]] |
| - | [[Category: Ribosome]]
| + | [[Category: Wienk H]] |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Translation initiation]]
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| Structural highlights
Function
IF2_GEOSE One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Publication Abstract from PubMed
Bacterial translation initiation factor IF2 complexed with GTP binds to the 30S ribosomal subunit, promotes ribosomal binding of fMet-tRNA, and favors the joining of the small and large ribosomal subunits yielding a 70S initiation complex ready to enter the translation elongation phase. Within the IF2 molecule subdomain G3, which is believed to play an important role in the IF2-30S interaction, is positioned between the GTP-binding G2 and the fMet-tRNA binding C-terminal subdomains. In this study the solution structure of subdomain G3 of Geobacillus stearothermophilus IF2 has been elucidated. G3 forms a core structure consisting of two beta-sheets with each four anti-parallel strands, followed by a C-terminal alpha-helix. In line with its role as linker between G3 and subdomain C1, this helix has no well-defined orientation but is endowed with a dynamic nature. The structure of the G3 core is that of a typical OB-fold module, similar to that of the corresponding subdomain of Thermus thermophilus IF2, and to that of other known RNA-binding modules such as IF2-C2, IF1 and subdomains II of elongation factors EF-Tu and EF-G. Structural comparisons have resulted in a model that describes the interaction between IF2-G3 and the 30S ribosomal subunit.
A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit.,Dongre R, Folkers GE, Gualerzi CO, Boelens R, Wienk H Protein Sci. 2016 Jul 1. doi: 10.1002/pro.2977. PMID:27364543[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dongre R, Folkers GE, Gualerzi CO, Boelens R, Wienk H. A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit. Protein Sci. 2016 Jul 1. doi: 10.1002/pro.2977. PMID:27364543 doi:http://dx.doi.org/10.1002/pro.2977
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