This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2p2k

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of a lectin from Canavalia gladiata seeds (CGL) in complex with man1-4man-OMe

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p2k"

@@ Line 1: / Line 1: @@
-[[Image:2p2k.jpg|left|200px]]
- {{Structure
+==Crystal structure of a lectin from Canavalia gladiata seeds (CGL) in complex with man1-4man-OMe==
-|PDB= 2p2k |SIZE=350|CAPTION= <scene name='initialview01'>2p2k</scene>, resolution 1.98&Aring;
+<StructureSection load='2p2k' size='340' side='right'caption='[[2p2k]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+<table><tr><td colspan='2'>[[2p2k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P2K FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p2k OCA], [https://pdbe.org/2p2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p2k RCSB], [https://www.ebi.ac.uk/pdbsum/2p2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p2k ProSAT]</span></td></tr>
-|RELATEDENTRY=[[2ovu|2OVU]], [[2ow4|2OW4]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p2k OCA], [http://www.ebi.ac.uk/pdbsum/2p2k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p2k RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/CONA_CANGL CONA_CANGL] Glucose/D-mannose/rhamnose specific lectin. Has hemagglutinating activity towards rabbit erythrocytes. Has mitogenic activity towards murine splenocytes that is inhibited by glucose. Inhibits HIV-1 reverse transcriptase with an IC(50) of 35uM. Has a potent antiproliferative activity against L1210 leukemia cells in vitro that is not inhibited by glucose. Inhibits translation in cell-free rabbit reticulocyte system with an IC(50) of 2.08uM. Lacks anti-fungal activity against M.arachidicola, B.cenera and F.oxysporum.<ref>PMID:15935326</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p2/2p2k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p2k ConSurf].
+<div style="clear:both"></div>
-'''Crystal structure of a lectin from Canavalia gladiata seeds (CGL) in complex with man1-4man-OMe'''
+==See Also==
+*[[Concanavalin 3D structures|Concanavalin 3D structures]]
+== References ==
-==Overview==
+<references/>
-Plant lectins, especially those purified from species of the Leguminosae family, represent the best studied group of carbohydrate-binding proteins. The legume lectins from Diocleinae subtribe are highly similar proteins that present significant differences in the potency/efficacy of their biological activities. The structural studies of the interactions between lectins and sugars may clarify the origin of the distinct biological activities observed in this high similar class of proteins. In this way, this work presents a crystallographic study of the ConM and CGL (agglutinins from Canavalia maritima and Canavalia gladiata, respectively) in the following complexes: ConM/CGL:Man(alpha1-2)Man(alpha1-O)Me, ConM/CGL:Man(alpha1-3)Man(alpha1-O)Me and ConM/CGL:Man(alpha1-4)Man(alpha1-O)Me, which crystallized in different conditions and space group from the native proteins. The structures were solved by molecular replacement, presenting satisfactory values for R(factor) and R(free). Comparisons between ConM, CGL and ConA (Canavalia ensiformis lectin) binding mode with the dimannosides in subject, presented different interactions patterns, which may account for a structural explanation of the distincts biological properties observed in the lectins of Diocleinae subtribe.
+__TOC__
+</StructureSection>
-==About this Structure==
-P2K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2K OCA].
-==Reference==
-Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure-biological activity relationship in legume lectins., Bezerra GA, Oliveira TM, Moreno FB, de Souza EP, da Rocha BA, Benevides RG, Delatorre P, de Azevedo WF Jr, Cavada BS, J Struct Biol. 2007 Nov;160(2):168-76. Epub 2007 Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17881248 17881248]
 [[Category: Canavalia gladiata]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Benevides, G B.]]
+[[Category: Benevides GB]]
-[[Category: Bezerra, G A.]]
+[[Category: Bezerra GA]]
-[[Category: Cavada, B S.]]
+[[Category: Cavada BS]]
-[[Category: Delatorre, P.]]
+[[Category: Delatorre P]]
-[[Category: Jr., W F.de Azevedo.]]
+[[Category: Moreno FBMB]]
-[[Category: Moreno, F B.M B.]]
+[[Category: Oliveira TM]]
-[[Category: Oliveira, T M.]]
+[[Category: Rocha BAM]]
-[[Category: Rocha, B A.M.]]
+[[Category: Souza EP]]
-[[Category: Souza, E P.]]
+[[Category: De Azevedo Jr WF]]
-[[Category: canavalia gladiata lectin]]
-[[Category: dimannoside]]
-[[Category: man1-4man-ome]]
-[[Category: sugar binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:27:41 2008''

2p2k

From Proteopedia

Current revision

Crystal structure of a lectin from Canavalia gladiata seeds (CGL) in complex with man1-4man-OMe

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox