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2mwn

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Talin-F3 / RIAM N-terminal Peptide complex

Structural highlights

2mwn is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AB1IP_HUMAN Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.^[1] ^[2]

Publication Abstract from PubMed

The membrane localization and activation of cytoskeletal protein talin are key steps to initiate the integrin transmembrane receptors' activation, which mediates many cellular adhesive responses such as cell migration, spreading and proliferation. RIAM, a membrane anchor and small GTPase RAP1 effector, is known to bind to the C-terminal rod domain of talin (talin-R) and promote localizations of talin to the membrane. Through systematic mapping analysis, we find that RIAM also binds to the N-terminal head of talin (talin-H), a crucial domain involved in binding and activating integrins. We show that the RIAM binding to talin-H sterically occludes the binding of a talin-R domain that otherwise masks the integrin-binding site on talin-H. We further provide functional evidence that such RIAM-mediated steric unmasking of talin triggers integrin activation. Our findings thus uncover a novel role for RIAM in conformational regulation of talin during integrin activation and cell adhesion.

Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion.,Yang J, Zhu L, Zhang H, Hirbawi J, Fukuda K, Dwivedi P, Liu J, Byzova T, Plow EF, Wu J, Qin J Nat Commun. 2014 Dec 18;5:5880. doi: 10.1038/ncomms6880. PMID:25520155^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Inagaki T, Suzuki S, Miyamoto T, Takeda T, Yamashita K, Komatsu A, Yamauchi K, Hashizume K. The retinoic acid-responsive proline-rich protein is identified in promyeloleukemic HL-60 cells. J Biol Chem. 2003 Dec 19;278(51):51685-92. Epub 2003 Oct 6. PMID:14530287 doi:10.1074/jbc.M308016200
↑ Lafuente EM, van Puijenbroek AA, Krause M, Carman CV, Freeman GJ, Berezovskaya A, Constantine E, Springer TA, Gertler FB, Boussiotis VA. RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev Cell. 2004 Oct;7(4):585-95. PMID:15469846 doi:10.1016/j.devcel.2004.07.021
↑ Yang J, Zhu L, Zhang H, Hirbawi J, Fukuda K, Dwivedi P, Liu J, Byzova T, Plow EF, Wu J, Qin J. Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion. Nat Commun. 2014 Dec 18;5:5880. doi: 10.1038/ncomms6880. PMID:25520155 doi:http://dx.doi.org/10.1038/ncomms6880

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mwn"

@@ Line 1: / Line 1: @@
 ==Talin-F3 / RIAM N-terminal Peptide complex==
-<StructureSection load='2mwn' size='340' side='right' caption='[[2mwn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mwn' size='340' side='right'caption='[[2mwn]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MWN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWN FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TLN1, KIAA1027, TLN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwn OCA], [http://pdbe.org/2mwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mwn RCSB], [http://www.ebi.ac.uk/pdbsum/2mwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwn OCA], [https://pdbe.org/2mwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwn RCSB], [https://www.ebi.ac.uk/pdbsum/2mwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/AB1IP_HUMAN AB1IP_HUMAN]] Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.<ref>PMID:14530287</ref> <ref>PMID:15469846</ref>  [[http://www.uniprot.org/uniprot/TLN1_HUMAN TLN1_HUMAN]] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity).
+[https://www.uniprot.org/uniprot/AB1IP_HUMAN AB1IP_HUMAN] Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.<ref>PMID:14530287</ref> <ref>PMID:15469846</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Talin|Talin]]
+*[[Talin 3D structures|Talin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Byzova, T]]
+[[Category: Large Structures]]
-[[Category: Dwivedi, P]]
+[[Category: Byzova T]]
-[[Category: Fukuda, K]]
+[[Category: Dwivedi P]]
-[[Category: Hirbawi, J]]
+[[Category: Fukuda K]]
-[[Category: Liu, J]]
+[[Category: Hirbawi J]]
-[[Category: Plow, E F]]
+[[Category: Liu J]]
-[[Category: Qin, J]]
+[[Category: Plow EF]]
-[[Category: Wu, J]]
+[[Category: Qin J]]
-[[Category: Yang, J]]
+[[Category: Wu J]]
-[[Category: Zhang, H]]
+[[Category: Yang J]]
-[[Category: Zhu, L]]
+[[Category: Zhang H]]
-[[Category: Integrin]]
+[[Category: Zhu L]]
-[[Category: Riam]]
-[[Category: Structural protein-signaling protein complex]]
-[[Category: Talin]]

2mwn

From Proteopedia

Current revision

Talin-F3 / RIAM N-terminal Peptide complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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