Phosphocarrier protein

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<StructureSection load='' size='350' side='right' caption='Structure of phosphocarrier protein HPr (PDB entry [[1fu0]])' scene='57/570591/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Structure of phosphocarrier protein HPr (PDB entry [[1fu0]])' scene='57/570591/Cv/1'>
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__TOC__
== Function ==
== Function ==
'''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system. The system catalyses the phosphorylation of sugars during their translocation across cell membrane. A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>. A conserved histidine in the N terminal of HPr is the acceptor of the phosphate. In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated.
'''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system. The system catalyses the phosphorylation of sugars during their translocation across cell membrane. A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>. A conserved histidine in the N terminal of HPr is the acceptor of the phosphate. In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated.
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== Structural highlights ==
== Structural highlights ==
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The <scene name='57/570591/Cv/2'>phosphorylation of Ser46</scene> prohibits the HPr to act as a phosphocarrier in the EI reaction<ref>PMID:11054290</ref>.
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The <scene name='57/570591/Cv/3'>phosphorylation of Ser46</scene> prohibits the HPr to act as a phosphocarrier in the EI reaction<ref>PMID:11054290</ref>.
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</StructureSection>
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==3D structures of phosphocarrier protein HPr==
==3D structures of phosphocarrier protein HPr==
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[[Phosphocarrier protein HPr 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Phosphocarrier protein HPr
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**[[2hpr]] - BsHPr (mutant) – ''Bacillus subtilis'' <br />
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**[[2hid]] - BsHPr (mutant) – NMR <br />
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**[[1jem]] - BsHPr (mutant) with phosphohistidine – NMR <br />
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**[[1sph]] – BsHPr <br />
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**[[1poh]] – EcHPr – ''Escherichia coli'' <br />
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**[[1opd]], [[1cm2]], [[1cm3]], [[3ccd]] - EcHPr (mutant) <br />
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**[[1hdn]] - EcHPr – NMR <br />
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**[[1pfh]] - EcHPr with phosphohistidine – NMR <br />
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**[[1ptf]] - EfHPr – ''Enterococcus faecalis'' <br />
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**[[1qfr]] - EfHPr – NMR <br />
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**[[1fu0]] - EfHPr with phosphoserine <br />
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**[[1pch]] – HPr – ''Mycoplasma capricolum''<br />
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**[[1qr5]] - ScHPr – ''Staphylococcus carnosus'' - NMR <br />
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**[[1txe]] - ScHPr (mutant) – NMR <br />
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**[[1ka5]] - HPr – ''Staphylococcus aureus'' - NMR <br />
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**[[1y4y]] – GsHPr – ''Geobacillus stearothermophilus''<br />
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**[[1y50]], [[1y51]] - GsHPr (mutant) <br />
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**[[3ihs]] – HPr – ''Bacillus anthracis'' <br />
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*Phosphocarrier protein HPr complex
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**[[2jel]] - EcHPr + antibody <br />
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**[[3eze]], [[3ezb]], [[3eza]] - EcHPr + EI N terminal + PO3 – NMR <br />
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**[[2xdf]] - EcHPr + EI N terminal – NMR <br />
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**[[1ggr]], [[1j6t]], [[1vrc]] - EcHPr + EII N terminal + PO3 – NMR <br />
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**[[2lrk]], [[2lrl]] - EcHPr + EII N terminal – NMR <br />
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**[[4xwj]] - EcHPr + antisigma factor RSD <br />
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**[[5ya0]] - EcHPr + autoinducer-2 kinase <br />
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**[[5ya1]] - EcHPr + autoinducer-2 kinase + ATP<br />
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**[[5ya2]] - EcHPr + autoinducer-2 kinase + ADP<br />
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**[[1kkl]] - BsHPr (mutant) + HPrK <br />
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**[[1kkm]] - BsHPr (mutant) with phosphoserine + HPrK/P <br />
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**[[2fep]] - BsHPr with phosphoserine + BsCcpA <br />
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**[[1rzr]] – BmHPr with phosphoserine + BmCcpA + DNA - ''Bacillus megaterium''<br />
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**[[2oen]] - BmHPr with phosphoserine + BmCcpA<br />
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**[[2nzu]] - BmHPr with phosphoserine + BmCcpA + glucose-6-phosphate <br />
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**[[2nzv]] - BmHPr with phosphoserine + BmCcpA + fructose-1,6-diphosphate <br />
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**[[3oqm]], [[3oqn]], [[3oqo]] - BsHPr with phosphoserine + BsCcpA + DNA<br />
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*Phosphocarrier protein NPr
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**[[5t17]], [[5t1n]] - EcNPr – NMR <br />
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**[[5t1o]] - EcNPr + EIN-NTR – NMR <br />
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*Phosphocarrier protein III Glc
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**[[1f3g]] - EcPrcpIII <br />
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of phosphocarrier protein HPr (PDB entry 1fu0)

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References

  1. ↑ Postma PW, Lengeler JW, Jacobson GR. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev. 1993 Sep;57(3):543-94. PMID:8246840
  2. ↑ Li X, Peterkofsky A, Wang G. Solution structure of NPr, a bacterial signal-transducing protein that controls the phosphorylation state of the potassium transporter-regulating protein IIA Ntr. Amino Acids. 2008 Oct;35(3):531-9. doi: 10.1007/s00726-008-0079-9. Epub 2008 Apr , 18. PMID:18421563 doi:http://dx.doi.org/10.1007/s00726-008-0079-9
  3. ↑ Maurer T, Doker R, Gorler A, Hengstenberg W, Kalbitzer HR. Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution. Eur J Biochem. 2001 Feb;268(3):635-44. PMID:11168402
  4. ↑ Audette GF, Engelmann R, Hengstenberg W, Deutscher J, Hayakawa K, Quail JW, Delbaere LT. The 1.9 A resolution structure of phospho-serine 46 HPr from Enterococcus faecalis. J Mol Biol. 2000 Nov 3;303(4):545-53. PMID:11054290 doi:10.1006/jmbi.2000.4166

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