6eer
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of glycine-bound GoxA from Pseudoalteromonas luteoviolacea== | |
| + | <StructureSection load='6eer' size='340' side='right'caption='[[6eer]], [[Resolution|resolution]] 1.82Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6eer]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_luteoviolacea_DSM_6061 Pseudoalteromonas luteoviolacea DSM 6061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EER FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=TNQ:6-[(carboxymethyl)amino]-7-hydroxy-L-tryptophan'>TNQ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eer OCA], [https://pdbe.org/6eer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eer RCSB], [https://www.ebi.ac.uk/pdbsum/6eer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eer ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A161XU12_9GAMM A0A161XU12_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The LodA-like proteins are a recently identified family of enzymes that rely on a cysteine tryptophylquinone (CTQ)1 cofactor for catalysis. They differ from other tryptophylquinone enzymes in that they are oxidases rather than dehydrogenases. GoxA is a member of this family that catalyzes the oxidative deamination of glycine. Our previous work with GoxA from Pseudoalteromonas luteoviolaceae demonstrated that this protein forms a stable intermediate upon anaerobic incubation with glycine. The spectroscopic properties of this species were unique among those identified for tryptophylquinone enzymes characterized to date. Here we use X-ray crystallography and resonance Raman spectroscopy to identify the GoxA catalytic intermediate as a product Schiff-base. Structural work additionally highlights features of the active site pocket that confer substrate specificity, intermediate stabilization and catalytic activity. The unusual properties of GoxA are discussed within the context of the other tryptophylquinone enzymes. | ||
| - | + | Structural and spectroscopic characterization of a product Schiff-base intermediate in the reaction of the quinoprotein glycine oxidase, GoxA.,Avalos D, Sabuncu S, Mamounis KJ, Davidson VL, Moenne-Loccoz P, Yukl ET Biochemistry. 2019 Jan 3. doi: 10.1021/acs.biochem.8b01145. PMID:30605596<ref>PMID:30605596</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Avalos | + | <div class="pdbe-citations 6eer" style="background-color:#fffaf0;"></div> |
| - | [[Category: Yukl | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudoalteromonas luteoviolacea DSM 6061]] | ||
| + | [[Category: Avalos D]] | ||
| + | [[Category: Yukl ET]] | ||
Current revision
Structure of glycine-bound GoxA from Pseudoalteromonas luteoviolacea
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