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2p6p

From Proteopedia

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[[Image:2p6p.jpg|left|200px]]
 
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{{Structure
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==X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2==
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|PDB= 2p6p |SIZE=350|CAPTION= <scene name='initialview01'>2p6p</scene>, resolution 1.88&Aring;
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<StructureSection load='2p6p' size='340' side='right'caption='[[2p6p]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[2p6p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6P FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
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|GENE= urdGT2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1906 Streptomyces fradiae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6p OCA], [https://pdbe.org/2p6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6p RCSB], [https://www.ebi.ac.uk/pdbsum/2p6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6p ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6p OCA], [http://www.ebi.ac.uk/pdbsum/2p6p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p6p RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q9RPA7_STRFR Q9RPA7_STRFR]
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== Evolutionary Conservation ==
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'''X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6p_consurf.spt"</scriptWhenChecked>
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The glycosyltransferase UrdGT2 from Streptomyces fradiae catalyzes the formation of a glycosidic C-C bond between a polyketide aglycone and D-olivose. The enyzme was expressed in Escherichia coli, purified and crystallized. Its structure was established by X-ray diffraction at 1.9 A resolution. It is the first structure of a C-glycosyltransferase. UrdGT2 belongs to the structural family GT-B of the glycosyltransferases and is likely to form a C(2)-symmetric dimer in solution. The binding structures of donor and acceptor substrates in five structurally homologous enzymes provided a clear and consistent guide for the substrate-binding structure in UrdGT2. The modeled substrate locations suggest the deeply buried Asp137 as the activator for C-C bond formation and explain the reaction. The putative model can be used to design mutations that change the substrate specificity. Such mutants are of great interest in overcoming the increasing danger of antibiotic resistance.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2P6P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6P OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6p ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin., Mittler M, Bechthold A, Schulz GE, J Mol Biol. 2007 Sep 7;372(1):67-76. Epub 2007 Jun 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17640665 17640665]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Streptomyces fradiae]]
[[Category: Streptomyces fradiae]]
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[[Category: Bechthold, A.]]
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[[Category: Bechthold A]]
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[[Category: Mittler, M.]]
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[[Category: Mittler M]]
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[[Category: Schulz, G E.]]
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[[Category: Schulz GE]]
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[[Category: c-glycosyltransferase,dtdp-d-olivose-transferase,polyketide aglycon]]
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[[Category: gt-b family]]
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[[Category: x-ray-diffraction,urdamycina-biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:30:56 2008''
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Current revision

X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2

PDB ID 2p6p

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