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| - | [[Image:2p73.jpg|left|200px]] | |
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| - | {{Structure
| + | ==crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1== |
| - | |PDB= 2p73 |SIZE=350|CAPTION= <scene name='initialview01'>2p73</scene>, resolution 2.300Å
| + | <StructureSection load='2p73' size='340' side='right'caption='[[2p73]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene> | + | <table><tr><td colspan='2'>[[2p73]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P73 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | |GENE= A64R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p73 OCA], [https://pdbe.org/2p73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p73 RCSB], [https://www.ebi.ac.uk/pdbsum/2p73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p73 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2p6w|2P6W]], [[2p72|2P72]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p73 OCA], [http://www.ebi.ac.uk/pdbsum/2p73 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p73 RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1] |
| - | | + | __TOC__ |
| - | '''crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Paramecium bursaria Chlorella virus 1]] |
| - | ==Overview==
| + | [[Category: Rossmann MG]] |
| - | Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
| + | [[Category: Van Etten JL]] |
| - | | + | [[Category: Xiang Y]] |
| - | ==About this Structure==
| + | [[Category: Zhang Y]] |
| - | 2P73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA].
| + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850743 17850743]
| + | |
| - | [[Category: Paramecium bursaria chlorella virus 1]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Etten, J L.Van.]] | + | |
| - | [[Category: Rossmann, M G.]] | + | |
| - | [[Category: Xiang, Y.]] | + | |
| - | [[Category: Zhang, Y.]] | + | |
| - | [[Category: crystal structure]]
| + | |
| - | [[Category: glycosyltransferase]]
| + | |
| - | [[Category: pbcv-1 a64r]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:31:12 2008''
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