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| | ==Beta-galactosidase== | | ==Beta-galactosidase== |
| - | <StructureSection load='6drv' size='340' side='right' caption='[[6drv]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <SX load='6drv' size='340' side='right' viewer='molstar' caption='[[6drv]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6drv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DRV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6drv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DRV FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacZ, b0344, JW0335 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6drv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6drv OCA], [https://pdbe.org/6drv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6drv RCSB], [https://www.ebi.ac.uk/pdbsum/6drv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6drv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6drv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6drv OCA], [http://pdbe.org/6drv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6drv RCSB], [http://www.ebi.ac.uk/pdbsum/6drv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6drv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BGAL_ECOLI BGAL_ECOLI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6drv" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6drv" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Galactosidase 3D structures|Galactosidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Beta-galactosidase]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Ecoli]] | + | [[Category: Large Structures]] |
| - | [[Category: Cianfrocco, M A]] | + | [[Category: Cianfrocco MA]] |
| - | [[Category: DiMaio, F]] | + | [[Category: DiMaio F]] |
| - | [[Category: Lahiri, I]] | + | [[Category: Lahiri I]] |
| - | [[Category: Leschziner, A E]] | + | [[Category: Leschziner AE]] |
| - | [[Category: Amazon web service]]
| + | |
| - | [[Category: Cryo-em]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Relion]]
| + | |
| Structural highlights
Function
BGAL_ECOLI
Publication Abstract from PubMed
Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2A structure of ss-galactosidase in approximately 55h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud.
cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.,Cianfrocco MA, Lahiri I, DiMaio F, Leschziner AE J Struct Biol. 2018 Jun 1. pii: S1047-8477(18)30138-2. doi:, 10.1016/j.jsb.2018.05.014. PMID:29864529[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cianfrocco MA, Lahiri I, DiMaio F, Leschziner AE. cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud. J Struct Biol. 2018 Jun 1. pii: S1047-8477(18)30138-2. doi:, 10.1016/j.jsb.2018.05.014. PMID:29864529 doi:http://dx.doi.org/10.1016/j.jsb.2018.05.014
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