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| | ==Restriction endonuclease MvaI in the absence of DNA== | | ==Restriction endonuclease MvaI in the absence of DNA== |
| - | <StructureSection load='2oa9' size='340' side='right' caption='[[2oa9]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='2oa9' size='340' side='right'caption='[[2oa9]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2oa9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15306 Atcc 15306]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OA9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2oa9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kocuria_varians Kocuria varians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OA9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2oaa|2oaa]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mvaIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1272 ATCC 15306])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oa9 OCA], [https://pdbe.org/2oa9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oa9 RCSB], [https://www.ebi.ac.uk/pdbsum/2oa9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oa9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oa9 OCA], [http://pdbe.org/2oa9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oa9 RCSB], [http://www.ebi.ac.uk/pdbsum/2oa9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2oa9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8RNV5_KOCVA Q8RNV5_KOCVA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 15306]] | + | [[Category: Kocuria varians]] |
| - | [[Category: Bochtler, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Czapinska, H]] | + | [[Category: Bochtler M]] |
| - | [[Category: Kaus-Drobek, M]] | + | [[Category: Czapinska H]] |
| - | [[Category: Siksnys, V]] | + | [[Category: Kaus-Drobek M]] |
| - | [[Category: Sokolowska, M]] | + | [[Category: Siksnys V]] |
| - | [[Category: Szczepanowski, R H]] | + | [[Category: Sokolowska M]] |
| - | [[Category: Tamulaitis, G]] | + | [[Category: Szczepanowski RH]] |
| - | [[Category: Urbanke, K]] | + | [[Category: Tamulaitis G]] |
| - | [[Category: Hydrolase]]
| + | [[Category: Urbanke K]] |
| - | [[Category: Monomeric endonuclease]]
| + | |
| - | [[Category: Mvai]]
| + | |
| - | [[Category: Restriction enzyme]]
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| Structural highlights
Function
Q8RNV5_KOCVA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Restriction endonuclease MvaI recognizes the sequence CC/WGG (W stands for A or T, '/' designates the cleavage site) and generates products with single nucleotide 5'-overhangs. The enzyme has been noted for its tolerance towards DNA modifications. Here, we report a biochemical characterization and crystal structures of MvaI in an apo-form and in a complex with target DNA at 1.5 A resolution. Our results show that MvaI is a monomer and recognizes its pseudosymmetric target sequence asymmetrically. The enzyme consists of two lobes. The catalytic lobe anchors the active site residues Glu36, Asp50, Glu55 and Lys57 and contacts the bases from the minor grove side. The recognition lobe mediates all major grove interactions with the bases. The enzyme in the crystal is bound to the strand with T at the center of the recognition sequence. The crystal structure with calcium ions and DNA mimics the prereactive state. MvaI shows structural similarities to BcnI, which cleaves the related sequence CC/SGG and to MutH enzyme, which is a component of the DNA repair machinery, and nicks one DNA strand instead of making a double-strand break.
Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically.,Kaus-Drobek M, Czapinska H, Sokolowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M Nucleic Acids Res. 2007;35(6):2035-46. Epub 2007 Mar 7. PMID:17344322[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kaus-Drobek M, Czapinska H, Sokolowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M. Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically. Nucleic Acids Res. 2007;35(6):2035-46. Epub 2007 Mar 7. PMID:17344322 doi:10.1093/nar/gkm064
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