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| | ==Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (P2221).== | | ==Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (P2221).== |
| - | <StructureSection load='2o6t' size='340' side='right' caption='[[2o6t]], [[Resolution|resolution]] 2.55Å' scene=''> | + | <StructureSection load='2o6t' size='340' side='right'caption='[[2o6t]], [[Resolution|resolution]] 2.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2o6t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O6T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2o6t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O6T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2av9|2av9]], [[2o5u|2o5u]], [[2o6b|2o6b]], [[2o6u|2o6u]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA5185 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o6t OCA], [https://pdbe.org/2o6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o6t RCSB], [https://www.ebi.ac.uk/pdbsum/2o6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o6t ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o6t OCA], [http://pdbe.org/2o6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o6t RCSB], [http://www.ebi.ac.uk/pdbsum/2o6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o6t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HU04_PSEAE Q9HU04_PSEAE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Thioesterase|Thioesterase]] | + | *[[Thioesterase 3D structures|Thioesterase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chruszcz, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Cymborowski, M]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Edwards, A]] | + | [[Category: Chruszcz M]] |
| - | [[Category: Evdokimova, E]] | + | [[Category: Cymborowski M]] |
| - | [[Category: Koclega, K D]] | + | [[Category: Edwards A]] |
| - | [[Category: Kudritska, M]] | + | [[Category: Evdokimova E]] |
| - | [[Category: Minor, W]] | + | [[Category: Koclega KD]] |
| - | [[Category: Savchenko, A]] | + | [[Category: Kudritska M]] |
| - | [[Category: Hot-dog fold]]
| + | [[Category: Minor W]] |
| - | [[Category: Hydrolase]]
| + | [[Category: Savchenko A]] |
| - | [[Category: Putative thioesterase]]
| + | |
| Structural highlights
Function
Q9HU04_PSEAE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of PA5185, a putative thioesterase from Pseudomonas aeruginosa strain PAO1, was solved using multi-wavelength anomalous diffraction to 2.4 A. Analysis of the structure and information about the putative function of the protein were used to optimize crystallization conditions. The crystal growth was optimized by applying additives with chemical similarity to a fragment of a putative PA5185 substrate (CoA or its derivative). Using new crystallization conditions containing this function-biased set of additives, several new crystal forms were produced and structures of three of them (in three different space groups) were determined. One of the new crystal forms had an improved resolution limit of 1.9 A, and another displayed an alternative conformation of the highly-conserved loop containing Asn26, which could play a physiological role. Surprisingly, none of the additives were ordered in the crystal structures. Application of function-biased additives could be used as a standard optimization protocol for producing improved diffraction, or new crystal forms, which may lead to better understanding of the biological functions of proteins.
Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1.,Chruszcz M, Zimmerman MD, Wang S, Koclega KD, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W Cryst Growth Des. 2008 Nov 5;8(11):4054-4061. PMID:19898606[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chruszcz M, Zimmerman MD, Wang S, Koclega KD, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W. Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1. Cryst Growth Des. 2008 Nov 5;8(11):4054-4061. PMID:19898606 doi:10.1021/cg800430f
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