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| | ==Xanthomonas XopD C470A Mutant== | | ==Xanthomonas XopD C470A Mutant== |
| - | <StructureSection load='2oix' size='340' side='right' caption='[[2oix]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='2oix' size='340' side='right'caption='[[2oix]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2oix]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_vesicatorium"_doidge_1921 "bacterium vesicatorium" doidge 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OIX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2oix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_euvesicatoria Xanthomonas euvesicatoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OIX FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2oiv|2oiv]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xopD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=456327 "Bacterium vesicatorium" Doidge 1921])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oix OCA], [https://pdbe.org/2oix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oix RCSB], [https://www.ebi.ac.uk/pdbsum/2oix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oix ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oix OCA], [http://pdbe.org/2oix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oix RCSB], [http://www.ebi.ac.uk/pdbsum/2oix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2oix ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q3BYJ5_XANC5 Q3BYJ5_XANC5] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium vesicatorium doidge 1921]] | + | [[Category: Large Structures]] |
| - | [[Category: Brautigam, C A]] | + | [[Category: Xanthomonas euvesicatoria]] |
| - | [[Category: Chosed, R]] | + | [[Category: Brautigam CA]] |
| - | [[Category: Machius, M]] | + | [[Category: Chosed R]] |
| - | [[Category: Orth, K]] | + | [[Category: Machius M]] |
| - | [[Category: Tomchick, D R]] | + | [[Category: Orth K]] |
| - | [[Category: Clan ce family 48 cysteine protease]]
| + | [[Category: Tomchick DR]] |
| - | [[Category: Desumoylating enzyme]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Isopeptidase activity]]
| + | |
| - | [[Category: Peptidase activity]]
| + | |
| - | [[Category: Secreted virulence facter]]
| + | |
| - | [[Category: Type iii secreted effector]]
| + | |
| Structural highlights
Function
Q3BYJ5_XANC5
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
XopD (Xanthomonas outer protein D), a type III secreted effector from Xanthomonas campestris pv. vesicatoria, is a desumoylating enzyme with strict specificity for its plant small ubiquitin-like modifier (SUMO) substrates. Based on SUMO sequence alignments and peptidase assays with various plant, yeast, and mammalian SUMOs, we identified residues in SUMO that contribute to XopD/SUMO recognition. Further predictions regarding the enzyme/substrate specificity were made by solving the XopD crystal structure. By incorporating structural information with sequence alignments and enzyme assays, we were able to elucidate determinants of the rigid SUMO specificity exhibited by the Xanthomonas virulence factor XopD.
Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases.,Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K J Biol Chem. 2007 Mar 2;282(9):6773-82. Epub 2007 Jan 3. PMID:17204475[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K. Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases. J Biol Chem. 2007 Mar 2;282(9):6773-82. Epub 2007 Jan 3. PMID:17204475 doi:10.1074/jbc.M608730200
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