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| | ==High pH structure of Rtms5 H146S variant== | | ==High pH structure of Rtms5 H146S variant== |
| - | <StructureSection load='2p4m' size='340' side='right' caption='[[2p4m]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='2p4m' size='340' side='right'caption='[[2p4m]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2p4m]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Coral Coral]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2P4M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2p4m]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Montipora_efflorescens Montipora efflorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P4M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mou|1mou]], [[1mov|1mov]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p4m OCA], [https://pdbe.org/2p4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p4m RCSB], [https://www.ebi.ac.uk/pdbsum/2p4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p4m ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rtms5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=105610 Coral])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p4m OCA], [http://pdbe.org/2p4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2p4m RCSB], [http://www.ebi.ac.uk/pdbsum/2p4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2p4m ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NFCP_MONEF NFCP_MONEF]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.<ref>PMID:12595737</ref> | + | [https://www.uniprot.org/uniprot/NFCP_MONEF NFCP_MONEF] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.<ref>PMID:12595737</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Coral]] | + | [[Category: Large Structures]] |
| - | [[Category: Battad, J M]] | + | [[Category: Montipora efflorescens]] |
| - | [[Category: Byres, E]] | + | [[Category: Battad JM]] |
| - | [[Category: Devenish, R J]] | + | [[Category: Byres E]] |
| - | [[Category: Dove, S G]] | + | [[Category: Devenish RJ]] |
| - | [[Category: Olsen, S]] | + | [[Category: Dove SG]] |
| - | [[Category: Prescott, M]] | + | [[Category: Olsen S]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Prescott M]] |
| - | [[Category: Smith, S C]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Turcic, K N]] | + | [[Category: Smith SC]] |
| - | [[Category: Wilmann, P G]] | + | [[Category: Turcic KN]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Wilmann PG]] |
| - | [[Category: Chromophore]]
| + | |
| - | [[Category: Chromoprotein]]
| + | |
| - | [[Category: Gfp-like protein]]
| + | |
| - | [[Category: Luminescent protein]]
| + | |
| Structural highlights
Function
NFCP_MONEF Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Within the fluorescent protein and chromoprotein family, the phenomenon of photoswitching is both intriguing and biotechnologically useful. Illumination of particular chromoproteins with intense light results in dramatic increases in fluorescence efficiency (termed kindling) and involves cis-trans isomerization of the chromophore. Here we report that chromophore isomerization can also be driven via alteration in pH. Specifically, we demonstrate that a number of naturally occurring chromoproteins, and their engineered variants, undergo a dramatic 20-100-fold increase in fluorescence efficiency at alkaline pH (>pH9.0). We have determined to 1.8 A resolution the structure of one such chromoprotein, Rtms5(H146S), in its highly far-red fluorescent form (Phi(F), 0.11 at pH 10.7) and compared it to the structure of the non-fluorescent form (Phi(F), 0.002 at pH 8.0). At high pH, the cyclic tri-peptide chromophore was observed to be mobile and distributed between a trans non-coplanar and a cis coplanar conformation, whereas at the lower pH, only a trans non-coplanar chromophore was observed. Calculation of pK(a) values suggested that titration of the side-chain of the conserved Glu215 close to the chromophore is involved in promoting the cis-coplanar conformation. Collectively, our data establish that isomerization to form a coplanar chromophore is a basis of the increased fluorescence efficiency at high pH. The phenomenon of pH-induced fluorescence gain has similarities with photoswitching, thereby providing a model to study the mechanism of kindling.
A structural basis for the pH-dependent increase in fluorescence efficiency of chromoproteins.,Battad JM, Wilmann PG, Olsen S, Byres E, Smith SC, Dove SG, Turcic KN, Devenish RJ, Rossjohn J, Prescott M J Mol Biol. 2007 May 11;368(4):998-1010. Epub 2007 Feb 9. PMID:17376484[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Beddoe T, Ling M, Dove S, Hoegh-Guldberg O, Devenish RJ, Prescott M, Rossjohn J. The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):597-9. Epub 2003, Feb 21. PMID:12595737
- ↑ Battad JM, Wilmann PG, Olsen S, Byres E, Smith SC, Dove SG, Turcic KN, Devenish RJ, Rossjohn J, Prescott M. A structural basis for the pH-dependent increase in fluorescence efficiency of chromoproteins. J Mol Biol. 2007 May 11;368(4):998-1010. Epub 2007 Feb 9. PMID:17376484 doi:10.1016/j.jmb.2007.02.007
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