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| | ==NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system== | | ==NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system== |
| - | <StructureSection load='2ofq' size='340' side='right' caption='[[2ofq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2ofq' size='340' side='right'caption='[[2ofq]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ofq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Incn_plasmid_r46 Incn plasmid r46]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ofq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/IncN_plasmid_R46 IncN plasmid R46]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">traO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46]), traN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofq OCA], [http://pdbe.org/2ofq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ofq RCSB], [http://www.ebi.ac.uk/pdbsum/2ofq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofq OCA], [https://pdbe.org/2ofq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofq RCSB], [https://www.ebi.ac.uk/pdbsum/2ofq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q79SE3_SALTM Q79SE3_SALTM] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Incn plasmid r46]] | + | [[Category: IncN plasmid R46]] |
| - | [[Category: Bayliss, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Driscoll, P C]] | + | [[Category: Bayliss R]] |
| - | [[Category: Harris, R]]
| + | [[Category: Driscoll PC]] |
| - | [[Category: Waksman, G]] | + | [[Category: Harris R]] |
| - | [[Category: Pkm101]] | + | [[Category: Waksman G]] |
| - | [[Category: Protein transport-protein transport complex]] | + | |
| - | [[Category: Tran]]
| + | |
| - | [[Category: Trao]]
| + | |
| Structural highlights
Function
Q79SE3_SALTM
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits and VirD4. Two subunits, VirB9 and VirB7, form an important stabilizing complex in the outer membrane. We describe here the NMR structure of a complex between the C-terminal domain of the VirB9 homolog TraO (TraO(CT)), bound to VirB7-like TraN from plasmid pKM101. TraO(CT) forms a beta-sandwich around which TraN winds. Structure-based mutations in VirB7 and VirB9 of A. tumefaciens show that the heterodimer interface is conserved. Opposite this interface, the TraO structure shows a protruding three-stranded beta-appendage, and here, we supply evidence that the corresponding region of VirB9 of A. tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system.
NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system.,Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:17244707[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G. NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:17244707
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