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| | ==Monomeric G-DNA tetraplex from human C-kit promoter== | | ==Monomeric G-DNA tetraplex from human C-kit promoter== |
| - | <StructureSection load='2o3m' size='340' side='right' caption='[[2o3m]], [[NMR_Ensembles_of_Models | 11 NMR models]]' scene=''> | + | <StructureSection load='2o3m' size='340' side='right'caption='[[2o3m]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2o3m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O3M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2o3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O3M FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a5p|2a5p]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3m OCA], [http://pdbe.org/2o3m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o3m RCSB], [http://www.ebi.ac.uk/pdbsum/2o3m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3m OCA], [https://pdbe.org/2o3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o3m RCSB], [https://www.ebi.ac.uk/pdbsum/2o3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| - | [[Category: Burge, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Kuryavyi, V V]] | + | [[Category: Burge S]] |
| - | [[Category: Neidle, S]]
| + | [[Category: Kuryavyi VV]] |
| - | [[Category: Patel, D J]] | + | [[Category: Neidle S]] |
| - | [[Category: Phan, A T]] | + | [[Category: Patel DJ]] |
| - | [[Category: 5-nucleotide snap-back loop]] | + | [[Category: Phan AT]] |
| - | [[Category: Broken strand topolog]] | + | |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Edgewise]]
| + | |
| - | [[Category: G-tetrad]]
| + | |
| - | [[Category: Parallel g-quadruplex]]
| + | |
| - | [[Category: Single-residue cha reversal]]
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| Structural highlights
Publication Abstract from PubMed
The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit.
Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter.,Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:17362008[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ. Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter. J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:17362008 doi:http://dx.doi.org/10.1021/ja068739h
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