4z7k

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==Crystal structure of CRISPR RNA processing endoribonuclease Cas6b==
==Crystal structure of CRISPR RNA processing endoribonuclease Cas6b==
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<StructureSection load='4z7k' size='340' side='right' caption='[[4z7k]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='4z7k' size='340' side='right'caption='[[4z7k]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4z7k]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metm5 Metm5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z7K FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4z7k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_maripaludis Methanococcus maripaludis] and [https://en.wikipedia.org/wiki/Methanococcus_maripaludis_C5 Methanococcus maripaludis C5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7K FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z7l|4z7l]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MmarC5_0767 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=402880 METM5])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7k OCA], [https://pdbe.org/4z7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7k RCSB], [https://www.ebi.ac.uk/pdbsum/4z7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7k ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7k OCA], [http://pdbe.org/4z7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z7k RCSB], [http://www.ebi.ac.uk/pdbsum/4z7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7k ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A4FXZ3_METM5 A4FXZ3_METM5]
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A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6.
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A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.,Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H Structure. 2016 Apr 5;24(4):547-554. doi: 10.1016/j.str.2016.02.009. Epub 2016, Mar 17. PMID:26996962<ref>PMID:26996962</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4z7k" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[CRISPR subtype I-B|CRISPR subtype I-B]]
*[[CRISPR subtype I-B|CRISPR subtype I-B]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Metm5]]
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[[Category: Large Structures]]
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[[Category: Li, H]]
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[[Category: Methanococcus maripaludis]]
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[[Category: Cas6]]
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[[Category: Methanococcus maripaludis C5]]
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[[Category: Crispr rna]]
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[[Category: Li H]]
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[[Category: Endoribonuclease]]
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[[Category: Hydrolase-rna-dna complex]]
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Current revision

Crystal structure of CRISPR RNA processing endoribonuclease Cas6b

PDB ID 4z7k

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