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| | ==Structure of Adeno-Associated virus serotype 8== | | ==Structure of Adeno-Associated virus serotype 8== |
| - | <StructureSection load='2qa0' size='340' side='right' caption='[[2qa0]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='2qa0' size='340' side='right'caption='[[2qa0]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2qa0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_8 Adeno-associated virus - 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QA0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2qa0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_-_8 Adeno-associated virus - 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QA0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=202813 Adeno-associated virus - 8])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qa0 OCA], [http://pdbe.org/2qa0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qa0 RCSB], [http://www.ebi.ac.uk/pdbsum/2qa0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qa0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qa0 OCA], [https://pdbe.org/2qa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qa0 RCSB], [https://www.ebi.ac.uk/pdbsum/2qa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qa0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8JQF8_9VIRU Q8JQF8_9VIRU] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Virus coat protein|Virus coat protein]] | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Adeno-associated virus - 8]] | | [[Category: Adeno-associated virus - 8]] |
| - | [[Category: Nam, H J]] | + | [[Category: Large Structures]] |
| - | [[Category: Beta-barrel]] | + | [[Category: Nam H-J]] |
| - | [[Category: Icosahedral virus]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Function
Q8JQF8_9VIRU
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Adeno-associated viruses (AAVs) are being developed as gene therapy vectors, and their efficacy could be improved by a detailed understanding of their viral capsid structures. AAV serotype 8 (AAV8) shows a significantly greater liver transduction efficiency than those of other serotypes, which has resulted in efforts to develop this virus as a gene therapy vector for hemophilia A and familial hypercholesterolemia. Pseudotyping studies show that the differential tissue tropism and transduction efficiencies exhibited by the AAVs result from differences in their capsid viral protein (VP) amino acids. Towards identifying the structural features underpinning these disparities, we report the crystal structure of the AAV8 viral capsid determined to 2.6-A resolution. The overall topology of its common overlapping VP is similar to that previously reported for the crystal structures of AAV2 and AAV4, with an eight-stranded beta-barrel and long loops between the beta-strands. The most significant structural differences between AAV8 and AAV2 (the best-characterized serotype) are located on the capsid surface at protrusions surrounding the two-, three-, and fivefold axes at residues reported to control transduction efficiency and antibody recognition for AAV2. In addition, a comparison of the AAV8 and AAV2 capsid surface amino acids showed a reduced distribution of basic charge for AAV8 at the mapped AAV2 heparin sulfate receptor binding region, consistent with an observed non-heparin-binding phenotype for AAV8. Thus, this AAV8 structure provides an additional platform for mutagenesis efforts to characterize AAV capsid regions responsible for differential cellular tropism, transduction, and antigenicity for these promising gene therapy vectors.
Structure of adeno-associated virus serotype 8, a gene therapy vector.,Nam HJ, Lane MD, Padron E, Gurda B, McKenna R, Kohlbrenner E, Aslanidi G, Byrne B, Muzyczka N, Zolotukhin S, Agbandje-McKenna M J Virol. 2007 Nov;81(22):12260-71. Epub 2007 Aug 29. PMID:17728238[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nam HJ, Lane MD, Padron E, Gurda B, McKenna R, Kohlbrenner E, Aslanidi G, Byrne B, Muzyczka N, Zolotukhin S, Agbandje-McKenna M. Structure of adeno-associated virus serotype 8, a gene therapy vector. J Virol. 2007 Nov;81(22):12260-71. Epub 2007 Aug 29. PMID:17728238 doi:http://dx.doi.org/10.1128/JVI.01304-07
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