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2pkm

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[[Image:2pkm.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of M tuberculosis Adenosine Kinase complexed with adenosine==
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|PDB= 2pkm |SIZE=350|CAPTION= <scene name='initialview01'>2pkm</scene>, resolution 1.90&Aring;
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<StructureSection load='2pkm' size='340' side='right'caption='[[2pkm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Adn+Binding+Site+For+Residue+A+501'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>
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<table><tr><td colspan='2'>[[2pkm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PKM FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= Rv2202 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkm OCA], [https://pdbe.org/2pkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pkm RCSB], [https://www.ebi.ac.uk/pdbsum/2pkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pkm ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1rka|1RKA]], [[1rkd|1RKD]], [[1bx4|1BX4]], [[2pkf|2PKF]], [[2pkk|2PKK]], [[2pkn|2PKN]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkm OCA], [http://www.ebi.ac.uk/pdbsum/2pkm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pkm RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ADOK_MYCTU ADOK_MYCTU] ATP dependent phosphorylation of adenosine to monophosphate derivatives (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pkm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pkm ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of M tuberculosis Adenosine Kinase complexed with adenosine'''
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==See Also==
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*[[Adenosine kinase 3D structures|Adenosine kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Adenosine kinase (ADK) catalyzes the phosphorylation of adenosine (Ado) to adenosine monophosphate (AMP). It is part of the purine salvage pathway that has been identified only in eukaryotes, with the single exception of Mycobacterium spp. Whereas it is not clear if Mycobacterium tuberculosis (Mtb) ADK is essential, it has been shown that the enzyme can selectively phosphorylate nucleoside analogs to produce products toxic to the cell. We have determined the crystal structure of Mtb ADK unliganded as well as ligand (Ado) bound at 1.5- and 1.9-A resolution, respectively. The structure of the binary complexes with the inhibitor 2-fluoroadenosine (F-Ado) bound and with the adenosine 5'-(beta,gamma-methylene)triphosphate (AMP-PCP) (non-hydrolyzable ATP analog) bound were also solved at 1.9-A resolution. These four structures indicate that Mtb ADK is a dimer formed by an extended beta sheet. The active site of the unliganded ADK is in an open conformation, and upon Ado binding a lid domain of the protein undergoes a large conformation change to close the active site. In the closed conformation, the lid forms direct interactions with the substrate and residues of the active site. Interestingly, AMP-PCP binding alone was not sufficient to produce the closed state of the enzyme. The binding mode of F-Ado was characterized to illustrate the role of additional non-bonding interactions in Mtb ADK compared with human ADK.
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[[Category: Large Structures]]
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==About this Structure==
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2PKM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKM OCA].
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==Reference==
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High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme., Reddy MC, Palaninathan SK, Shetty ND, Owen JL, Watson MD, Sacchettini JC, J Biol Chem. 2007 Sep 14;282(37):27334-42. Epub 2007 Jun 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17597075 17597075]
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[[Category: Adenosine kinase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Owen JL]]
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[[Category: Owen, J L.]]
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[[Category: Palaninathan SK]]
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[[Category: Palaninathan, S K.]]
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[[Category: Reddy MCM]]
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[[Category: Reddy, M C.M.]]
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[[Category: Sacchettini JC]]
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[[Category: Sacchettini, J C.]]
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[[Category: Shetty ND]]
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[[Category: Shetty, N D.]]
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[[Category: Watson MD]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Watson, M D.]]
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[[Category: adenosine]]
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[[Category: adenosine kinase]]
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[[Category: mycobacterium tuberculosis]]
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[[Category: structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:37:14 2008''
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Current revision

Crystal structure of M tuberculosis Adenosine Kinase complexed with adenosine

PDB ID 2pkm

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