6m9r

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'''Unreleased structure'''
 
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The entry 6m9r is ON HOLD
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==Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 with a bound N(delta)-hydroxy-N(omega)-methyl-L-arginine intermediate==
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<StructureSection load='6m9r' size='340' side='right'caption='[[6m9r]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6m9r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_achromogenes_subsp._streptozoticus Streptomyces achromogenes subsp. streptozoticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M9R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=J9Y:(2S)-2-amino-5-{[(E)-amino(methylamino)methylidene](hydroxy)azaniumyl}pentanoate'>J9Y</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9r OCA], [https://pdbe.org/6m9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m9r RCSB], [https://www.ebi.ac.uk/pdbsum/6m9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9r ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Small molecules containing the N-nitroso group, such as the bacterial natural product streptozotocin, are prominent carcinogens(1,2) and important cancer chemotherapeutics(3,4). Despite the considerable importance of this functional group to human health, enzymes dedicated to the assembly of the N-nitroso unit have not been identified. Here we show that SznF, a metalloenzyme from the biosynthesis of streptozotocin, catalyses an oxidative rearrangement of the guanidine group of N(omega)-methyl-L-arginine to generate an N-nitrosourea product. Structural characterization and mutagenesis of SznF reveal two separate active sites that promote distinct steps in this transformation using different iron-containing metallocofactors. This biosynthetic reaction, which has little precedent in enzymology or organic synthesis, expands the catalytic capabilities of non-haem-iron-dependent enzymes to include N-N bond formation. We find that biosynthetic gene clusters that encode SznF homologues are widely distributed among bacteria-including environmental organisms, plant symbionts and human pathogens-which suggests an unexpectedly diverse and uncharacterized microbial reservoir of bioactive N-nitroso metabolites.
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Authors: Rohac, R., Mitchell, A.J., Boal, A.K.
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An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin.,Ng TL, Rohac R, Mitchell AJ, Boal AK, Balskus EP Nature. 2019 Feb;566(7742):94-99. doi: 10.1038/s41586-019-0894-z. Epub 2019 Feb, 6. PMID:30728519<ref>PMID:30728519</ref>
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Description: Crystal Structure of N-nitrosating metalloenzyme SznF from S. achromogenes var. Strepzototicus in complex with N'-hydroxyl NG-methyl L-arginine
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Boal, A.K]]
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<div class="pdbe-citations 6m9r" style="background-color:#fffaf0;"></div>
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[[Category: Rohac, R]]
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== References ==
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[[Category: Mitchell, A.J]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces achromogenes subsp. streptozoticus]]
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[[Category: Boal AK]]
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[[Category: Mitchell AJ]]
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[[Category: Rohac R]]

Current revision

Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 with a bound N(delta)-hydroxy-N(omega)-methyl-L-arginine intermediate

PDB ID 6m9r

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