5zhx

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==Crystal structure of SmgGDS-558 and farnesylated RhoA complex==
==Crystal structure of SmgGDS-558 and farnesylated RhoA complex==
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<StructureSection load='5zhx' size='340' side='right' caption='[[5zhx]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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<StructureSection load='5zhx' size='340' side='right'caption='[[5zhx]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5zhx]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5zhx]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAR:FARNESYL'>FAR</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhx OCA], [http://pdbe.org/5zhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zhx RCSB], [http://www.ebi.ac.uk/pdbsum/5zhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhx ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAR:FARNESYL'>FAR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhx OCA], [https://pdbe.org/5zhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zhx RCSB], [https://www.ebi.ac.uk/pdbsum/5zhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GDS1_HUMAN GDS1_HUMAN]] Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. [[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
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[https://www.uniprot.org/uniprot/GDS1_HUMAN GDS1_HUMAN] Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SmgGDS has dual functions in cells and regulates small GTPases as both a guanine nucleotide exchange factor (GEF) for the Rho family and a molecular chaperone for small GTPases possessing a C-terminal polybasic region followed by four C-terminal residues called the CaaX motif, which is posttranslationally prenylated at its cysteine residue. Our recent structural work revealed that SmgGDS folds into tandem copies of armadillo-repeat motifs (ARMs) that are not present in other GEFs. However, the precise mechanism of GEF activity and recognition mechanism for the prenylated CaaX motif remain unknown because SmgGDS does not have a typical GEF catalytic domain and lacks a pocket to accommodate a prenyl group. Here, we aimed to determine the crystal structure of the SmgGDS/farnesylated RhoA complex. We found that SmgGDS induces a significant conformational change in the switch I and II regions that opens up the nucleotide-binding site, with the prenyl group fitting into the cryptic pocket in the N-terminal ARMs. Taken together, our findings could advance the understanding of the role of SmgGDS and enable drug design strategies for targeting SmgGDS and small GTPases.
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GEF mechanism revealed by the structure of SmgGDS-558 and farnesylated RhoA complex and its implication for a chaperone mechanism.,Shimizu H, Toma-Fukai S, Kontani K, Katada T, Shimizu T Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9563-9568. doi:, 10.1073/pnas.1804740115. Epub 2018 Sep 6. PMID:30190425<ref>PMID:30190425</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5zhx" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Rho GTPase 3D structures|Rho GTPase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Shimizu, H]]
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[[Category: Homo sapiens]]
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[[Category: Shimizu, T]]
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[[Category: Large Structures]]
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[[Category: Toma-Fukai, S]]
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[[Category: Shimizu H]]
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[[Category: Armadillo gef chaperone]]
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[[Category: Shimizu T]]
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[[Category: Oncoprotein]]
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[[Category: Toma-Fukai S]]

Current revision

Crystal structure of SmgGDS-558 and farnesylated RhoA complex

PDB ID 5zhx

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