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6gx6

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Crystal structure of IMP3 RRM12 in complex with RNA (ACAC)

Structural highlights

6gx6 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	6fq1, 6fqr
Gene:	IGF2BP3, IMP3, KOC1, VICKZ3 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF2B3_HUMAN] RNA-binding factor that may recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.^[1] ^[2]

Publication Abstract from PubMed

The IMP family of RNA binding proteins, also named as insulin-like growth factor 2 (IGF2) mRNA-binding proteins (IGF2BPs), are highly conserved RNA regulators that are involved in many RNA processing stages, including mRNA stability, localization and translation. There are three paralogs in the IMP family, IMP1-3, in mammals that all adopt the same domain arrangement with two RNA recognition motifs (RRM) in the N-terminus and four KH domains in the C-terminus. Here, we report the structure and biochemical characterization of IMP3 RRM12 and its complex with two short RNAs. These structures show that both RRM domains of IMP3 adopt the canonical RRM topology with two alpha-helices packed on an anti-parallel four stranded beta-sheet. The spatial orientation of RRM1 to RRM2 is unique compared with other known tandem RRM structures. In the IMP3 RRM12 complex with RNA, only RRM1 is involved in RNA binding and recognizes a dinucleotide sequence.

Structural basis of IMP3 RRM12 recognition of RNA.,Jia M, Gut H, Chao JA RNA. 2018 Aug 22. pii: rna.065649.118. doi: 10.1261/rna.065649.118. PMID:30135093^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vikesaa J, Hansen TV, Jonson L, Borup R, Wewer UM, Christiansen J, Nielsen FC. RNA-binding IMPs promote cell adhesion and invadopodia formation. EMBO J. 2006 Apr 5;25(7):1456-68. Epub 2006 Mar 16. PMID:16541107 doi:7601039
↑ Wachter K, Kohn M, Stohr N, Huttelmaier S. Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains. Biol Chem. 2013 Aug;394(8):1077-90. doi: 10.1515/hsz-2013-0111. PMID:23640942 doi:http://dx.doi.org/10.1515/hsz-2013-0111
↑ Jia M, Gut H, Chao JA. Structural basis of IMP3 RRM12 recognition of RNA. RNA. 2018 Aug 22. pii: rna.065649.118. doi: 10.1261/rna.065649.118. PMID:30135093 doi:http://dx.doi.org/10.1261/rna.065649.118

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6gx6"

@@ Line 3: / Line 3: @@
 <StructureSection load='6gx6' size='340' side='right' caption='[[6gx6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6gx6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6gx6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GX6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GX6 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6fq1|6fq1]], [[6fqr|6fqr]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IGF2BP3, IMP3, KOC1, VICKZ3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gx6 OCA], [http://pdbe.org/6gx6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gx6 RCSB], [http://www.ebi.ac.uk/pdbsum/6gx6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gx6 ProSAT]</span></td></tr>
 </table>
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Chao, A J]]
 [[Category: Gut, H]]

6gx6

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Current revision

Crystal structure of IMP3 RRM12 in complex with RNA (ACAC)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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