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| | ==PYP ultra-high resolution of a bacterial photoreceptor== | | ==PYP ultra-high resolution of a bacterial photoreceptor== |
| - | <StructureSection load='2qj7' size='340' side='right' caption='[[2qj7]], [[Resolution|resolution]] 1.05Å' scene=''> | + | <StructureSection load='2qj7' size='340' side='right'caption='[[2qj7]], [[Resolution|resolution]] 1.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2qj7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QJ7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2qj7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QJ7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qj5|2qj5]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pyp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qj7 OCA], [https://pdbe.org/2qj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qj7 RCSB], [https://www.ebi.ac.uk/pdbsum/2qj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qj7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qj7 OCA], [http://pdbe.org/2qj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qj7 RCSB], [http://www.ebi.ac.uk/pdbsum/2qj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qj7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA]] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response. | + | [https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Halorhodospira halophila]] | | [[Category: Halorhodospira halophila]] |
| - | [[Category: Coureux, P D]] | + | [[Category: Large Structures]] |
| - | [[Category: Fan, Z P]] | + | [[Category: Coureux PD]] |
| - | [[Category: Genick, U K]] | + | [[Category: Fan ZP]] |
| - | [[Category: Stojanoff, V]] | + | [[Category: Genick UK]] |
| - | [[Category: Pas domain]] | + | [[Category: Stojanoff V]] |
| - | [[Category: Pyp]]
| + | |
| - | [[Category: Signal transduction]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
PYP_HALHA Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Protein structural fluctuations occur over a wide spatial scale, ranging from minute, picometer-scale displacements, to large, interdomain motions and partial unfolding. While large-scale protein structural changes and their effects on protein function have been the focus of much recent attention, small-scale fluctuations have been less well studied, and are generally assumed to have proportionally smaller effects. Here we use the bacterial photoreceptor photoactive yellow protein (PYP) to test if subtle structural changes do, indeed, imply equally subtle functional effects. We flash froze crystals of PYP to trap the protein's conformational ensemble, and probed the molecules in this ensemble for their ability to facilitate PYP's biological function (i.e., light-driven isomerization of its chromophore). Our results indicate that the apparently homogeneous structural state observed in a 0.82 A crystal structure in fact comprises an ensemble of conformational states, in which subpopulations with nearly identical structures display dramatically different functional properties.
Picometer-scale conformational heterogeneity separates functional from nonfunctional states of a photoreceptor protein.,Coureux PD, Fan ZP, Stojanoff V, Genick UK Structure. 2008 Jun;16(6):863-72. PMID:18547519[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Coureux PD, Fan ZP, Stojanoff V, Genick UK. Picometer-scale conformational heterogeneity separates functional from nonfunctional states of a photoreceptor protein. Structure. 2008 Jun;16(6):863-72. PMID:18547519 doi:10.1016/j.str.2008.02.022
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