2qrf

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==Green Fluorescent Protein: Cyclized-only Intermediate of Chromophore Maturation in the Q183E variant==
==Green Fluorescent Protein: Cyclized-only Intermediate of Chromophore Maturation in the Q183E variant==
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<StructureSection load='2qrf' size='340' side='right' caption='[[2qrf]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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<StructureSection load='2qrf' size='340' side='right'caption='[[2qrf]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2qrf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2qrf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZO:[(2R,4S)-2-[(1S,2R)-1-AMINO-2-HYDROXYPROPYL]-2-HYDROXY-4-(4-HYDROXYBENZYL)-5-OXOIMIDAZOLIDIN-1-YL]ACETIC+ACID'>CZO</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZO:[(2R,4S)-2-[(1S,2R)-1-AMINO-2-HYDROXYPROPYL]-2-HYDROXY-4-(4-HYDROXYBENZYL)-5-OXOIMIDAZOLIDIN-1-YL]ACETIC+ACID'>CZO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qt2|2qt2]], [[2qz0|2qz0]], [[2awl|2awl]], [[2awm|2awm]], [[2awj|2awj]], [[1ema|1ema]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qrf OCA], [https://pdbe.org/2qrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qrf RCSB], [https://www.ebi.ac.uk/pdbsum/2qrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qrf ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qrf OCA], [http://pdbe.org/2qrf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qrf RCSB], [http://www.ebi.ac.uk/pdbsum/2qrf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qrf ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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<jmolCheckbox>
<jmolCheckbox>
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qrf_consurf.spt"</scriptWhenChecked>
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qrf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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==See Also==
==See Also==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aeqvi]]
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[[Category: Aequorea victoria]]
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[[Category: Barondeau, D P]]
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[[Category: Large Structures]]
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[[Category: Getzoff, E D]]
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[[Category: Barondeau DP]]
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[[Category: Hitomi, C]]
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[[Category: Getzoff ED]]
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[[Category: Tainer, J A]]
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[[Category: Hitomi C]]
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[[Category: Wood, T I]]
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[[Category: Tainer JA]]
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[[Category: Chromophore]]
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[[Category: Wood TI]]
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[[Category: Cyclized intermediate]]
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[[Category: Fluorescent protein]]
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[[Category: Gfp]]
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[[Category: Luminescence]]
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[[Category: Photoprotein]]
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Current revision

Green Fluorescent Protein: Cyclized-only Intermediate of Chromophore Maturation in the Q183E variant

PDB ID 2qrf

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