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| | ==Solution structure of the second RNA recognition motif (RRM) of TIA-1== | | ==Solution structure of the second RNA recognition motif (RRM) of TIA-1== |
| - | <StructureSection load='2rne' size='340' side='right' caption='[[2rne]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2rne' size='340' side='right'caption='[[2rne]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2rne]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2rne]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RNE FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tia1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rne OCA], [http://pdbe.org/2rne PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rne RCSB], [http://www.ebi.ac.uk/pdbsum/2rne PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rne ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rne OCA], [https://pdbe.org/2rne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rne RCSB], [https://www.ebi.ac.uk/pdbsum/2rne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rne ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TIA1_MOUSE TIA1_MOUSE] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Abe, C]] | + | [[Category: Mus musculus]] |
| - | [[Category: Guntert, P]] | + | [[Category: Abe C]] |
| - | [[Category: Hayashizaki, Y]] | + | [[Category: Guntert P]] |
| - | [[Category: Inoue, M]] | + | [[Category: Hayashizaki Y]] |
| - | [[Category: Kigawa, T]] | + | [[Category: Inoue M]] |
| - | [[Category: Kobayashi, N]] | + | [[Category: Kigawa T]] |
| - | [[Category: Kuwasako, K]] | + | [[Category: Kobayashi N]] |
| - | [[Category: Muto, Y]] | + | [[Category: Kuwasako K]] |
| - | [[Category: Shirouzu, M]] | + | [[Category: Muto Y]] |
| - | [[Category: Taguchi, S]] | + | [[Category: Shirouzu M]] |
| - | [[Category: Takahashi, M]] | + | [[Category: Taguchi S]] |
| - | [[Category: Tanaka, A]] | + | [[Category: Takahashi M]] |
| - | [[Category: Terada, T]] | + | [[Category: Tanaka A]] |
| - | [[Category: Tsuda, K]] | + | [[Category: Terada T]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Tsuda K]] |
| - | [[Category: Rna binding protein]]
| + | [[Category: Yokoyama S]] |
| - | [[Category: Rrm domain]]
| + | |
| Structural highlights
Function
TIA1_MOUSE Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
T cell intracellular antigen-1 (TIA-1), an apoptosis promoting factor, functions as a splicing regulator for the Fas pre-mRNA. TIA-1 possesses three RNA recognition motifs (RRMs) and a glutamine-rich domain. The second RRM (RRM2) is necessary and sufficient for tight, sequence-specific binding to the uridine-rich sequences buried around the 5' splice sites. In the present study, we solved the solution structure of the murine TIA-1 RRM2 by heteronuclear-nuclear magnetic resonance spectroscopy. The TIA-1 RRM2 adopts the RRM fold (betaalphabetabetaalphabeta) and possesses an extra beta-strand between beta2 and beta3, which forms an additional beta-sheet with the C-terminal part of beta2. We refer to this structure as the beta2-beta2' beta-loop. Interestingly, this characteristic beta-loop structure is conserved among a number of RRMs, including the U2AF65 RRM2 and the Sex-lethal RRM1 and RRM2, which also bind to uridine-rich RNAs. Furthermore, we identified a new sequence motif in the beta2-beta2' beta-loop, the DxxT motif. Chemical shift perturbation analyses of both the main and side chains upon binding to the uridine pentamer RNA revealed that most of the beta-sheet surface, including the beta2-beta2' beta-loop, is involved in the RNA binding. An investigation of the chemical shift perturbation revealed similarity in the RNA recognition modes between the TIA-1 and U2AF65 RRMs.
Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode.,Kuwasako K, Takahashi M, Tochio N, Abe C, Tsuda K, Inoue M, Terada T, Shirouzu M, Kobayashi N, Kigawa T, Taguchi S, Tanaka A, Hayashizaki Y, Guntert P, Muto Y, Yokoyama S Biochemistry. 2008 Jun 17;47(24):6437-50. Epub 2008 May 24. PMID:18500819[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kuwasako K, Takahashi M, Tochio N, Abe C, Tsuda K, Inoue M, Terada T, Shirouzu M, Kobayashi N, Kigawa T, Taguchi S, Tanaka A, Hayashizaki Y, Guntert P, Muto Y, Yokoyama S. Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode. Biochemistry. 2008 Jun 17;47(24):6437-50. Epub 2008 May 24. PMID:18500819 doi:10.1021/bi7024723
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