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| | ==Structure of antiplasmin== | | ==Structure of antiplasmin== |
| - | <StructureSection load='2r9y' size='340' side='right' caption='[[2r9y]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='2r9y' size='340' side='right'caption='[[2r9y]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2r9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R9Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2r9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R9Y FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Serpinf2, Pli ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9y OCA], [http://pdbe.org/2r9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r9y RCSB], [http://www.ebi.ac.uk/pdbsum/2r9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2r9y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9y OCA], [https://pdbe.org/2r9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r9y RCSB], [https://www.ebi.ac.uk/pdbsum/2r9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r9y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A2AP_MOUSE A2AP_MOUSE]] Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity). | + | [https://www.uniprot.org/uniprot/A2AP_MOUSE A2AP_MOUSE] Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Buckle, A M]] | + | [[Category: Mus musculus]] |
| - | [[Category: Coughlin, P B]] | + | [[Category: Buckle AM]] |
| - | [[Category: Hitchen, C R]] | + | [[Category: Coughlin PB]] |
| - | [[Category: Horvath, A J]] | + | [[Category: Hitchen CR]] |
| - | [[Category: Kan, W T]] | + | [[Category: Horvath AJ]] |
| - | [[Category: Langendorf, C G]] | + | [[Category: Kan WT]] |
| - | [[Category: Law, R H.P]] | + | [[Category: Langendorf CG]] |
| - | [[Category: Sofian, T]] | + | [[Category: Law RHP]] |
| - | [[Category: Whisstock, J C]] | + | [[Category: Sofian T]] |
| - | [[Category: Acute phase]]
| + | [[Category: Whisstock JC]] |
| - | [[Category: Alpha2-antiplasmin]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Inhibitory serpin]]
| + | |
| - | [[Category: Plasmin inhibitor]]
| + | |
| - | [[Category: Protease inhibitor]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Serine protease inhibitor]]
| + | |
| - | [[Category: Sulfation]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
A2AP_MOUSE Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The serpin alpha(2)-antiplasmin (SERPINF2) is the principal inhibitor of plasmin and inhibits fibrinolysis. Accordingly, alpha(2)-antiplasmin deficiency in humans results in uncontrolled fibrinolysis and a bleeding disorder. alpha(2)-antiplasmin is an unusual serpin, in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. To understand how this may happen, we have determined the 2.65A X-ray crystal structure of an N-terminal truncated murine alpha(2)-antiplasmin. The structure reveals that part of the C-terminal sequence is tightly associated with the body of the serpin. This would be anticipated to position the flexible plasmin-binding portion of the C-terminus in close proximity to the serpin Reactive Center Loop where it may act as a template to accelerate serpin/protease interactions.
X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin.,Law RH, Sofian T, Kan WT, Horvath AJ, Hitchen CR, Langendorf CG, Buckle AM, Whisstock JC, Coughlin PB Blood. 2008 Feb 15;111(4):2049-52. Epub 2007 Dec 6. PMID:18063751[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Law RH, Sofian T, Kan WT, Horvath AJ, Hitchen CR, Langendorf CG, Buckle AM, Whisstock JC, Coughlin PB. X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin. Blood. 2008 Feb 15;111(4):2049-52. Epub 2007 Dec 6. PMID:18063751 doi:10.1182/blood-2007-09-114215
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