6hk1
From Proteopedia
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(New page: '''Unreleased structure''' The entry 6hk1 is ON HOLD Authors: Engilberge, S., Wagner, T., Santoni, G., Breyton, C., Shima, S., Franzetti, B., Riobe, F., Maury, O., Girard, E. Descripti...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Thiazole synthase from Methanothermococcus thermolithotrophicus co-crystallized with Tb-Xo4== | |
| + | <StructureSection load='6hk1' size='340' side='right'caption='[[6hk1]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6hk1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HK1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=48F:[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+[(2R,3R)-2,3,5-TRIS(OXIDANYL)-4-OXIDANYLIDENE-PENTYL]+HYDROGEN+PHOSPHATE'>48F</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hk1 OCA], [http://pdbe.org/6hk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hk1 RCSB], [http://www.ebi.ac.uk/pdbsum/6hk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hk1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches. | ||
| - | + | Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.,Engilberge S, Wagner T, Santoni G, Breyton C, Shima S, Franzetti B, Riobe F, Maury O, Girard E J Appl Crystallogr. 2019 Jun 28;52(Pt 4):722-731. doi: 10.1107/S1600576719006381., eCollection 2019 Aug 1. PMID:31396026<ref>PMID:31396026</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6hk1" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Methanothermococcus thermolithotrophicus]] | ||
[[Category: Breyton, C]] | [[Category: Breyton, C]] | ||
| + | [[Category: Engilberge, S]] | ||
[[Category: Franzetti, B]] | [[Category: Franzetti, B]] | ||
| + | [[Category: Girard, E]] | ||
| + | [[Category: Maury, O]] | ||
| + | [[Category: Riobe, F]] | ||
[[Category: Santoni, G]] | [[Category: Santoni, G]] | ||
| - | [[Category: | + | [[Category: Shima, S]] |
| + | [[Category: Wagner, T]] | ||
| + | [[Category: Biosynthetic protein]] | ||
| + | [[Category: Crystallophore]] | ||
| + | [[Category: De novo phasing]] | ||
| + | [[Category: Lanthanide complex]] | ||
| + | [[Category: Molecular glue]] | ||
| + | [[Category: Native purification]] | ||
| + | [[Category: Nucleating agent]] | ||
| + | [[Category: Tb-xo4]] | ||
Current revision
Crystal structure of the Thiazole synthase from Methanothermococcus thermolithotrophicus co-crystallized with Tb-Xo4
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Categories: Large Structures | Methanothermococcus thermolithotrophicus | Breyton, C | Engilberge, S | Franzetti, B | Girard, E | Maury, O | Riobe, F | Santoni, G | Shima, S | Wagner, T | Biosynthetic protein | Crystallophore | De novo phasing | Lanthanide complex | Molecular glue | Native purification | Nucleating agent | Tb-xo4
